Detailed information of TA system TA165576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 774443..775353
• Replicon: chromosome
• Accession: NZ_CP057630
• Organism: Citrobacter sp. RHB20-C16

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HVX64_RS03865
• Protein ID: WP_004883378.1
• Coordinates: 774883..775353 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: HVX64_RS03860
• Protein ID: WP_032413443.1
• Coordinates: 774443..774886 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX64_RS03840	769595..771031	-	1437	WP_023288222.1	3-isopropylmalate dehydratase large subunit	-
HVX64_RS03845	771161..772393	-	1233	WP_023288221.1	MFS transporter	-
HVX64_RS03855	774113..774342	+	230	Protein_757	DUF4113 domain-containing protein	-
HVX64_RS03860	774443..774886	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
HVX64_RS03865	774883..775353	+	471	WP_004883378.1	RES family NAD+ phosphorylase	Toxin
HVX64_RS03870	775591..776559	-	969	WP_181825287.1	IS5-like element IS903B family transposase	-
HVX64_RS03875	776828..778363	-	1536	WP_181825288.1	MFS transporter	-
HVX64_RS03880	778366..779508	-	1143	WP_181825289.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	772873..776559	3686

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17500.82 Da
• Isoelectric Point: 4.6155

>T165576 WP_004883378.1 NZ_CP057630:774883-775353 [Citrobacter sp. RHB20-C16]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVLKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T165576 NZ_CP073929:1025966-1026301 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT165576 WP_032413443.1 NZ_CP057630:774443-774886 [Citrobacter sp. RHB20-C16]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT165576 NZ_CP073929:1025718-1025966 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4