Detailed information of TA system TA165574

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 84973..85830
• Replicon: chromosome
• Accession: NZ_CP057630
• Organism: Citrobacter sp. RHB20-C16

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7L6YXS7
• Locus tag: HVX64_RS00395
• Protein ID: WP_046401692.1
• Coordinates: 84973..85353 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HVX64_RS00400
• Protein ID: WP_080343259.1
• Coordinates: 85411..85830 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX64_RS00370	81007..81219	+	213	WP_044254506.1	DUF1471 domain-containing protein	-
HVX64_RS00375	81238..82335	+	1098	WP_181825209.1	alkene reductase	-
HVX64_RS00380	82506..82997	+	492	WP_044254500.1	GNAT family N-acetyltransferase	-
HVX64_RS00385	83325..84176	-	852	WP_090050165.1	DUF4942 domain-containing protein	-
HVX64_RS00390	84485..84976	-	492	WP_046401693.1	hypothetical protein	-
HVX64_RS00395	84973..85353	-	381	WP_046401692.1	TA system toxin CbtA family protein	Toxin
HVX64_RS00400	85411..85830	-	420	WP_080343259.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HVX64_RS00405	85799..86020	-	222	WP_038633763.1	DUF987 domain-containing protein	-
HVX64_RS00410	86032..86517	-	486	WP_038642868.1	DNA repair protein RadC	-
HVX64_RS00415	86570..86965	-	396	WP_181825417.1	antirestriction protein	-
HVX64_RS00420	87043..87306	-	264	WP_001149870.1	hypothetical protein	-
HVX64_RS00425	87306..88124	-	819	WP_001234374.1	DUF945 domain-containing protein	-
HVX64_RS00430	88240..88490	-	251	Protein_85	DUF905 family protein	-
HVX64_RS00435	88681..89148	-	468	WP_000727137.1	hypothetical protein	-
HVX64_RS00440	89245..89646	-	402	WP_000819262.1	hypothetical protein	-
HVX64_RS00445	89852..90739	-	888	WP_040229917.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	69490..99453	29963

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (33-132)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14234.49 Da
• Isoelectric Point: 10.6224

>T165574 WP_046401692.1 NZ_CP057630:c85353-84973 [Citrobacter sp. RHB20-C16]
MQTLSAIPTRKAPSRPTPVEIWQQLLTYLLKRHYGLSLSDTQFSDEKIITQYIDAGISLSDALNFLVEKSELVRIDRPGF
SIKHQSPFIGVIDILRARRATGLMQRNGYKRITLLIAGNAAQKQHS

Nucleotide

• Length: 381 bp

>T165574 NZ_CP073929:745648-745944 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15460.52 Da
• Isoelectric Point: 7.4130

>AT165574 WP_080343259.1 NZ_CP057630:c85830-85411 [Citrobacter sp. RHB20-C16]
MSFCAASPSVNSYSRSTTLLNKTLPANHHISEPWWGLKRNITPCFGARLVQEGNRLHYLADRASITGTFTDADLRHLDQA
FPALLKQLELMLVSGELNPRHQHCVTLYAKGLTCEADSLGSHGYIYTAIYPTPDDSITR

Nucleotide

• Length: 420 bp

>AT165574 NZ_CP073929:745946-746341 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A7L6YXS7