Detailed information of TA system TA165573

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yfjZ/CbtA-CbeA
• Location: 3955282..3956005
• Replicon: chromosome
• Accession: NZ_CP057620
• Organism: Citrobacter sp. RHB21-C01

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A481Q9T8
• Locus tag: HVX68_RS18845
• Protein ID: WP_053266278.1
• Coordinates: 3955282..3955602 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HVX68_RS18850
• Protein ID: WP_105607669.1
• Coordinates: 3955670..3956005 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX68_RS18825	3951404..3953251	+	1848	WP_042289358.1	RNA polymerase sigma factor RpoD	-
HVX68_RS18830	3953341..3953847	-	507	WP_060570862.1	G/U mismatch-specific DNA glycosylase	-
HVX68_RS18840	3954254..3954880	+	627	WP_181822089.1	serine acetyltransferase	-
HVX68_RS18845	3955282..3955602	-	321	WP_053266278.1	TA system toxin CbtA family protein	Toxin
HVX68_RS18850	3955670..3956005	-	336	WP_105607669.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HVX68_RS18855	3956019..3956498	-	480	WP_053266275.1	DNA repair protein RadC	-
HVX68_RS18860	3956510..3956959	-	450	WP_181822090.1	antirestriction protein	-
HVX68_RS18865	3957059..3957298	-	240	WP_053266273.1	DUF905 domain-containing protein	-
HVX68_RS18870	3957375..3957842	-	468	WP_053266281.1	hypothetical protein	-
HVX68_RS18875	3957866..3958309	-	444	WP_047620715.1	hypothetical protein	-
HVX68_RS18880	3958309..3958545	-	237	WP_001144031.1	hypothetical protein	-
HVX68_RS18885	3958586..3959287	-	702	WP_047620708.1	WYL domain-containing protein	-
HVX68_RS18890	3959504..3960325	-	822	WP_181822091.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12096.82 Da
• Isoelectric Point: 6.4799

>T165573 WP_053266278.1 NZ_CP057620:c3955602-3955282 [Citrobacter sp. RHB21-C01]
MQISTVPATMPVSSRLSPVQVWQQLLTYLLEHHYGLTLNDTPFHDDAAIKEHIEAGITLADAVNFLVERYELVRTDRKGF
TWQEQTPFVTATDILRARRATGLMNK

Nucleotide

• Length: 321 bp

>T165573 NZ_CP073929:681074-681388 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12594.36 Da
• Isoelectric Point: 7.0262

>AT165573 WP_105607669.1 NZ_CP057620:c3956005-3955670 [Citrobacter sp. RHB21-C01]
MYKDATPTWGLRRDITPCFGARLVQEGHRLHFLANRAGFTGSFSEVQTLQLNEAFPHFVAYLELMLLSCELNPRYAHCVT
LYRNGLTCEADTLGSHGHVYMAIYPSNQVKD

Nucleotide

• Length: 336 bp

>AT165573 NZ_CP073929:681385-681801 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGCTCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A481Q9T8