Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2664349..2664875 Replicon chromosome
Accession NZ_CP057620
Organism Citrobacter sp. RHB21-C01

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HVX68_RS12855 Protein ID WP_000323025.1
Coordinates 2664588..2664875 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HVX68_RS12850 Protein ID WP_000534858.1
Coordinates 2664349..2664588 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX68_RS12815 2659968..2660249 - 282 WP_181821503.1 phage holin family protein -
HVX68_RS12820 2660236..2660631 - 396 WP_042291608.1 phage holin family protein -
HVX68_RS12835 2661707..2663047 + 1341 WP_181821504.1 ISNCY family transposase -
HVX68_RS12840 2663484..2663816 - 333 WP_001317460.1 FlxA-like family protein -
HVX68_RS12845 2664019..2664324 - 306 WP_001326990.1 hypothetical protein -
HVX68_RS12850 2664349..2664588 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HVX68_RS12855 2664588..2664875 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HVX68_RS12860 2664974..2665783 - 810 WP_181821505.1 antitermination protein -
HVX68_RS12865 2665783..2665920 - 138 WP_181821506.1 YlcG family protein -
HVX68_RS12870 2665917..2666273 - 357 WP_181821507.1 RusA family crossover junction endodeoxyribonuclease -
HVX68_RS12875 2666270..2666551 - 282 WP_181821508.1 hypothetical protein -
HVX68_RS12880 2666751..2667356 - 606 WP_181821509.1 DUF1367 family protein -
HVX68_RS12885 2667441..2667662 - 222 WP_181821510.1 hypothetical protein -
HVX68_RS12890 2667772..2668005 - 234 WP_181821511.1 DinI-like family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2607228..2727943 120715


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T165563 WP_000323025.1 NZ_CP057620:2664588-2664875 [Citrobacter sp. RHB21-C01]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T165563 NZ_CP073926:2750952-2751059 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT165563 WP_000534858.1 NZ_CP057620:2664349-2664588 [Citrobacter sp. RHB21-C01]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT165563 NZ_CP073926:c2750905-2750839 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References