Detailed information of TA system TA165559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3299972..3300592
• Replicon: chromosome
• Accession: NZ_CP057611
• Organism: Citrobacter sp. RHB21-C05

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: HVX72_RS15835
• Protein ID: WP_001280991.1
• Coordinates: 3300374..3300592 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A0V9JYC1
• Locus tag: HVX72_RS15830
• Protein ID: WP_042285841.1
• Coordinates: 3299972..3300346 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX72_RS15820	3295112..3296305	+	1194	WP_042285835.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HVX72_RS15825	3296328..3299477	+	3150	WP_042285838.1	multidrug efflux RND transporter permease subunit	-
HVX72_RS15830	3299972..3300346	+	375	WP_042285841.1	Hha toxicity modulator TomB	Antitoxin
HVX72_RS15835	3300374..3300592	+	219	WP_001280991.1	hemolysin expression modulator Hha	Toxin
HVX72_RS15840	3300770..3301327	+	558	WP_042285845.1	maltose O-acetyltransferase	-
HVX72_RS15845	3301435..3301905	+	471	WP_042285848.1	YlaC family protein	-
HVX72_RS15850	3301953..3302093	-	141	WP_016537667.1	type B 50S ribosomal protein L36	-
HVX72_RS15855	3302096..3302356	-	261	WP_042285853.1	type B 50S ribosomal protein L31	-
HVX72_RS15860	3302535..3304085	+	1551	WP_181821859.1	EAL domain-containing protein	-
HVX72_RS15865	3304125..3304478	-	354	WP_060569050.1	DUF1428 family protein	-
HVX72_RS15870	3304559..3305155	-	597	WP_181821860.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T165559 WP_001280991.1 NZ_CP057611:3300374-3300592 [Citrobacter sp. RHB21-C05]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T165559 NZ_CP073926:1895174-1895428 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14467.25 Da
• Isoelectric Point: 4.7077

>AT165559 WP_042285841.1 NZ_CP057611:3299972-3300346 [Citrobacter sp. RHB21-C05]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWQKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT165559 NZ_CP073926:1894926-1895177 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 4ICG

Antitoxin

• AlphaFold DB: A0A0V9JYC1