Detailed information of TA system TA165549

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 755745..756399
• Replicon: chromosome
• Accession: NZ_CP057611
• Organism: Citrobacter sp. RHB21-C05

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: HVX72_RS03615
• Protein ID: WP_181822564.1
• Coordinates: 755992..756399 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A0V9JTE1
• Locus tag: HVX72_RS03610
• Protein ID: WP_042292140.1
• Coordinates: 755745..756011 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX72_RS03585	750942..752375	-	1434	WP_181822561.1	6-phospho-beta-glucosidase	-
HVX72_RS03590	752493..753221	-	729	WP_060569471.1	MurR/RpiR family transcriptional regulator	-
HVX72_RS03595	753274..753585	+	312	WP_042292149.1	N(4)-acetylcytidine aminohydrolase	-
HVX72_RS03600	753748..754407	+	660	WP_181822562.1	hemolysin III family protein	-
HVX72_RS03605	754508..755488	-	981	WP_181822563.1	tRNA-modifying protein YgfZ	-
HVX72_RS03610	755745..756011	+	267	WP_042292140.1	FAD assembly factor SdhE	Antitoxin
HVX72_RS03615	755992..756399	+	408	WP_181822564.1	protein YgfX	Toxin
HVX72_RS03620	756444..756965	-	522	WP_042292135.1	flavodoxin FldB	-
HVX72_RS03625	757065..757961	+	897	WP_181823236.1	site-specific tyrosine recombinase XerD	-
HVX72_RS03630	757985..758698	+	714	WP_181822565.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HVX72_RS03635	758704..760437	+	1734	WP_181822566.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15928.91 Da
• Isoelectric Point: 10.5720

>T165549 WP_181822564.1 NZ_CP057611:755992-756399 [Citrobacter sp. RHB21-C05]
VVLWQSDLRVSWRAQWLSLLLHGLVAAAILLMPWPLSYTPLWMILLSLVVFDCVRSQRRINTCQGEVKLLLDGRLRWQGR
EWTIVSAPWIIKTGMMLRLRSESGKRQHLWLAADSMDEAEWRDLCRLMLQQSMQR

Nucleotide

• Length: 408 bp

>T165549 NZ_CP073926:323384-323614 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10558.09 Da
• Isoelectric Point: 5.1496

>AT165549 WP_042292140.1 NZ_CP057611:755745-756011 [Citrobacter sp. RHB21-C05]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDDEKRIFVRLLECDDPDLFNWLMNHGKPADVELEQMVRLIQTRN
RDRGPVAI

Nucleotide

• Length: 267 bp

>AT165549 NZ_CP073926:323611-323970 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9JTE1