Detailed information of TA system TA165548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 253441..254072
• Replicon: chromosome
• Accession: NZ_CP057611
• Organism: Citrobacter sp. RHB21-C05

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HVX72_RS01100
• Protein ID: WP_181822379.1
• Coordinates: 253441..253716 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7L6Y5R2
• Locus tag: HVX72_RS01105
• Protein ID: WP_042286652.1
• Coordinates: 253713..254072 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX72_RS01090	249448..252192	+	2745	WP_181822378.1	ribosome-associated ATPase/putative transporter RbbA	-
HVX72_RS01095	252192..253316	+	1125	WP_042286658.1	ABC transporter permease	-
HVX72_RS01100	253441..253716	+	276	WP_181822379.1	type II toxin-antitoxin system HicA family toxin	Toxin
HVX72_RS01105	253713..254072	+	360	WP_042286652.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HVX72_RS01110	254154..254555	-	402	WP_042286649.1	nickel-responsive transcriptional regulator NikR	-
HVX72_RS01115	254568..255368	-	801	WP_181822380.1	nickel import ATP-binding protein NikE	-
HVX72_RS01120	255365..256129	-	765	WP_042286643.1	nickel import ATP-binding protein NikD	-
HVX72_RS01125	256129..256962	-	834	WP_042286641.1	nickel ABC transporter permease subunit NikC	-
HVX72_RS01130	256959..257903	-	945	WP_042286639.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10337.12 Da
• Isoelectric Point: 10.6850

>T165548 WP_181822379.1 NZ_CP057611:253441-253716 [Citrobacter sp. RHB21-C05]
MRQQVLSLRKKQRNMLEQLFKTPVPQAIKWLDIESLVKALGGEIKEGRGSRCKFLLNNSIACFHRPHPSPDTDKGAVESV
RDWLISIGVKP

Nucleotide

• Length: 276 bp

>T165548 NZ_CP073924:c4620950-4620639 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13271.98 Da
• Isoelectric Point: 4.7582

>AT165548 WP_042286652.1 NZ_CP057611:253713-254072 [Citrobacter sp. RHB21-C05]
MIKPKTPNAMEIAGHPAVIHYVPELNAFRGKFLGLSGHCDFVSDSIQGLQEEGEISLNEYLADCREAGIEPYAHTEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWIIDTLSERLKQA

Nucleotide

• Length: 360 bp

>AT165548 NZ_CP073924:c4620638-4620348 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7L6Y5R2