Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 63419..64020 Replicon plasmid pRHB23-C01_3
Accession NZ_CP057567
Organism Escherichia fergusonii strain RHB23-C01

Toxin (Protein)


Gene name doc Uniprot ID A0A7A7LY66
Locus tag HVX91_RS22695 Protein ID WP_001216036.1
Coordinates 63419..63799 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A765X875
Locus tag HVX91_RS22700 Protein ID WP_032271830.1
Coordinates 63799..64020 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX91_RS22670 58860..60344 - 1485 WP_000124159.1 hypothetical protein -
HVX91_RS22675 60344..61537 - 1194 WP_000219618.1 hypothetical protein -
HVX91_RS22680 61623..62075 - 453 WP_023352520.1 hypothetical protein -
HVX91_RS22685 62164..63207 - 1044 WP_032313385.1 DUF968 domain-containing protein -
HVX91_RS22690 63235..63414 - 180 WP_001448367.1 hypothetical protein -
HVX91_RS22695 63419..63799 - 381 WP_001216036.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HVX91_RS22700 63799..64020 - 222 WP_032271830.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HVX91_RS22705 64093..64482 - 390 WP_000506730.1 DNA repair protein -
HVX91_RS22710 64657..65229 + 573 WP_001133670.1 hypothetical protein -
HVX91_RS22715 65236..65487 - 252 WP_001667237.1 DNA polymerase III subunit theta -
HVX91_RS22720 66150..66500 - 351 WP_032313386.1 hypothetical protein -
HVX91_RS22725 66504..67436 - 933 WP_089621505.1 hypothetical protein -
HVX91_RS22730 67418..67792 - 375 WP_000988654.1 hypothetical protein -
HVX91_RS22735 67799..68092 - 294 WP_000269005.1 hypothetical protein -
HVX91_RS22740 68271..68504 - 234 WP_000516536.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..95926 95926


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13613.39 Da        Isoelectric Point: 5.6343

>T165546 WP_001216036.1 NZ_CP057567:c63799-63419 [Escherichia fergusonii]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELVDLTVGAATGEISVSTVAATLRRLYGSAE

Download         Length: 381 bp

>T165546 NZ_CP073924:c4234149-4233799 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8161.16 Da        Isoelectric Point: 5.1106

>AT165546 WP_032271830.1 NZ_CP057567:c64020-63799 [Escherichia fergusonii]
MRSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT165546 NZ_CP073924:c4234394-4234143 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7A7LY66

Antitoxin

Source ID Structure
AlphaFold DB A0A765X875

References