Detailed information of TA system TA165500

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3550232..3550886
• Replicon: chromosome
• Accession: NZ_CP057561
• Organism: Escherichia fergusonii strain RHB23-C02

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: F0JPE9
• Locus tag: HVX92_RS16990
• Protein ID: WP_000244774.1
• Coordinates: 3550232..3550639 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: HVX92_RS16995
• Protein ID: WP_181811890.1
• Coordinates: 3550620..3550886 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX92_RS16970	3546189..3547922	-	1734	WP_000813234.1	single-stranded-DNA-specific exonuclease RecJ	-
HVX92_RS16975	3547928..3548638	-	711	WP_000715223.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HVX92_RS16980	3548663..3549559	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
HVX92_RS16985	3549671..3550192	+	522	WP_001055874.1	flavodoxin FldB	-
HVX92_RS16990	3550232..3550639	-	408	WP_000244774.1	protein YgfX	Toxin
HVX92_RS16995	3550620..3550886	-	267	WP_181811890.1	FAD assembly factor SdhE	Antitoxin
HVX92_RS17000	3551138..3552118	+	981	WP_181811891.1	tRNA-modifying protein YgfZ	-
HVX92_RS17005	3552195..3552854	-	660	WP_000250283.1	hemolysin III family protein	-
HVX92_RS17010	3553018..3553329	-	312	WP_001182961.1	N(4)-acetylcytidine aminohydrolase	-
HVX92_RS17015	3553374..3554807	+	1434	WP_148047390.1	6-phospho-beta-glucosidase BglA	-
HVX92_RS17020	3554855..3555748	-	894	WP_000819362.1	transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16078.05 Da
• Isoelectric Point: 11.2511

>T165500 WP_000244774.1 NZ_CP057561:c3550639-3550232 [Escherichia fergusonii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T165500 NZ_CP073906:4047732-4047950 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10565.15 Da
• Isoelectric Point: 5.1599

>AT165500 WP_181811890.1 NZ_CP057561:c3550886-3550620 [Escherichia fergusonii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAEMEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT165500 NZ_CP073906:4047331-4047705 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0L6ZX35