Detailed information of TA system TA165469

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1209596..1210275
• Replicon: chromosome
• Accession: NZ_CP057509
• Organism: Citrobacter freundii strain RHB24-C03

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HVY04_RS05760
• Protein ID: WP_181820051.1
• Coordinates: 1209596..1209937 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HVY04_RS05765
• Protein ID: WP_181820052.1
• Coordinates: 1209958..1210275 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY04_RS05725	1204757..1205389	-	633	WP_117342165.1	fimbria biosynthesis transcriptional regulator FimZ	-
HVY04_RS05730	1205980..1206702	-	723	WP_181820048.1	fimbriae Y protein	-
HVY04_RS05735	1206725..1207450	-	726	WP_181820049.1	EAL domain-containing protein	-
HVY04_RS05740	1207615..1208208	-	594	WP_115259165.1	fimbria biosynthesis transcriptional regulator FimW	-
HVY04_RS05745	1208246..1208425	+	180	WP_115259164.1	hypothetical protein	-
HVY04_RS05755	1208644..1209480	-	837	WP_181820050.1	DUF4942 domain-containing protein	-
HVY04_RS05760	1209596..1209937	-	342	WP_181820051.1	TA system toxin CbtA family protein	Toxin
HVY04_RS05765	1209958..1210275	-	318	WP_181820052.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HVY04_RS05770	1210294..1210515	-	222	WP_181820053.1	DUF987 domain-containing protein	-
HVY04_RS05775	1210524..1211000	-	477	WP_088583106.1	RadC family protein	-
HVY04_RS05780	1211016..1211474	-	459	WP_181820054.1	antirestriction protein	-
HVY04_RS05785	1211571..1211810	-	240	WP_181820055.1	DUF905 domain-containing protein	-
HVY04_RS05790	1211965..1212438	-	474	WP_181820056.1	hypothetical protein	-
HVY04_RS05795	1212460..1212942	-	483	Protein_1133	GntR family transcriptional regulator	-
HVY04_RS05800	1213159..1213980	-	822	WP_181820057.1	DUF945 domain-containing protein	-
HVY04_RS05805	1214071..1214934	-	864	WP_181820326.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13001.98 Da
• Isoelectric Point: 10.4208

>T165469 WP_181820051.1 NZ_CP057509:c1209937-1209596 [Citrobacter freundii]
MKPQSAATSRAVKPCLTPVAVWQMLLTRLLEQHYGLTLNDTPFIDETVIQNHINAGITLADAVNFLVDKYELVRIDRRRF
SWQEQSPYLRAVDILRARQATGLLRQNRNNAVR

Nucleotide

• Length: 342 bp

>T165469 NZ_CP073893:c1893444-1893049 [Staphylococcus epidermidis]
ATGCAAAGCACTAAATATTTAACTGAAAAACAAGTGATTGCCATTAATGTAAAAGCAATACAAGATTTCTCACCAAAAGA
ACAAGTTGGTGTTAAAGTTCCAGAAGTTCTTAATGCTACTATTGAAGGAGTTAAACAATCATTCGGTGGAGTTGAACTAT
ATGAAACAATCGAGAGAAAAGCAGCTTTTATATATAGAAATATAGCTCAAAAGCACGCATTCCATAATGCGAATAAAAGA
ACAGCTTTTACATCTATGGTAATATTTTTGAAGTTAAATAGTATTAATTTTGAATGCACTCAAGATGAAGCGGTTCAGTT
TACTTTAAGAGTAGTAAACGACAAAAATCTTACTCTTGAGGGAATTGAGACATGGATCAAGAGGCATTGTAAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11984.61 Da
• Isoelectric Point: 7.3232

>AT165469 WP_181820052.1 NZ_CP057509:c1210275-1209958 [Citrobacter freundii]
MSNITWGLQRDITPRLGARLVQERNRLHYLADRASITGKFSDAECQKLDETFPHFISQMESMLTTGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAVYPTKR

Nucleotide

• Length: 318 bp

>AT165469 NZ_CP073893:c1893674-1893444 [Staphylococcus epidermidis]
ATGATTACTACAAGAAAACTAAGAAAAGCTGGTAATAGTTCAGTTGTTTCTGTACCGACAGAAGTGATCGCAGCACTCGG
AATATCAAATGGTGATAATCTTAAATTTAATGTAAAAGATAATAAAGTAACCATCGAAAAGGAGGTAAGAGAGGACGAGG
AGTTTTTTAAATTGTTGGATGAAACATTCACTGAGTATAACCAAGCTTTAAAAAGGATGGTTGACTTATAA