Detailed information of TA system TA165464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4292922..4293457
• Replicon: chromosome
• Accession: NZ_CP057506
• Organism: Citrobacter freundii strain RHB24-C05

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HVY06_RS20230
• Protein ID: WP_181819770.1
• Coordinates: 4293170..4293457 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D4BKM9
• Locus tag: HVY06_RS20225
• Protein ID: WP_006688247.1
• Coordinates: 4292922..4293173 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY06_RS20195	4287934..4288782	+	849	WP_135912711.1	alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-lyase PhnJ	-
HVY06_RS20200	4288779..4289537	+	759	WP_115259672.1	phosphonate C-P lyase system protein PhnK	-
HVY06_RS20205	4289646..4290326	+	681	WP_181819767.1	phosphonate C-P lyase system protein PhnL	-
HVY06_RS20210	4290323..4291459	+	1137	WP_096758663.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
HVY06_RS20215	4291462..4292007	+	546	WP_181819768.1	ribose 1,5-bisphosphokinase	-
HVY06_RS20220	4292080..4292838	+	759	WP_181819769.1	phosphonate metabolism protein PhnP	-
HVY06_RS20225	4292922..4293173	+	252	WP_006688247.1	plasmid stabilization protein	Antitoxin
HVY06_RS20230	4293170..4293457	+	288	WP_181819770.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVY06_RS20235	4293454..4293783	-	330	WP_096758667.1	hypothetical protein	-
HVY06_RS20240	4293852..4296116	-	2265	WP_181819771.1	hybrid sensor histidine kinase/response regulator	-
HVY06_RS20245	4296231..4297754	+	1524	WP_096758669.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11143.98 Da
• Isoelectric Point: 10.1604

>T165464 WP_181819770.1 NZ_CP057506:4293170-4293457 [Citrobacter freundii]
MSYTVKFREDALKEWQKLDKSIQQQFAKELKKCGENPHVPSAKLRGIKDCYIIQLRTSRFRLVYQVIDDTLVIAVVAVGK
RERSEVYNLASERLR

Nucleotide

• Length: 288 bp

>T165464 NZ_CP073890:682179-682388 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9215.53 Da
• Isoelectric Point: 4.2965

>AT165464 WP_006688247.1 NZ_CP057506:4292922-4293173 [Citrobacter freundii]
MPHIILSDTTASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQQLVKLVHERSQQPLVDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT165464 NZ_CP073890:681879-682172 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A7X1BT32