Detailed information of TA system TA165451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1137967..1138587
• Replicon: chromosome
• Accession: NZ_CP057506
• Organism: Citrobacter freundii strain RHB24-C05

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: HVY06_RS05430
• Protein ID: WP_002892050.1
• Coordinates: 1137967..1138185 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HVY06_RS05435
• Protein ID: WP_115259201.1
• Coordinates: 1138213..1138587 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY06_RS05400	1133797..1134408	+	612	WP_146717226.1	hypothetical protein	-
HVY06_RS05405	1134491..1134844	+	354	WP_115259217.1	DUF1428 family protein	-
HVY06_RS05410	1134898..1136451	-	1554	WP_115259216.1	EAL domain-containing protein	-
HVY06_RS05415	1136636..1136896	+	261	WP_115259215.1	type B 50S ribosomal protein L31	-
HVY06_RS05420	1136898..1137038	+	141	WP_005125190.1	type B 50S ribosomal protein L36	-
HVY06_RS05425	1137112..1137585	-	474	WP_096758041.1	YlaC family protein	-
HVY06_RS05430	1137967..1138185	-	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
HVY06_RS05435	1138213..1138587	-	375	WP_115259201.1	Hha toxicity modulator TomB	Antitoxin
HVY06_RS05440	1139076..1142225	-	3150	WP_096758039.1	efflux RND transporter permease AcrB	-
HVY06_RS05445	1142248..1143441	-	1194	WP_096758038.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T165451 WP_002892050.1 NZ_CP057506:c1138185-1137967 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T165451 NZ_CP073881:822389-822784 [Staphylococcus epidermidis]
ATGCAAAGCACTAAATATTTAACTGAAAAACAAGTGATTGCCATTAATGTAAAAGCAATACAAGATTTCTCACCAAAAGA
ACAAGTTGGTGTTAAAGTTCCAGAAGTTCTTAATGCTACTATTGAAGGAGTTAAACAATCATTCGGTGGAGTTGAACTAT
ATGAAACAATCGAGAGAAAAGCAGCTTTTATATATAGAAATATAGCTCAAAAGCACGCATTCCATAATGCGAATAAAAGA
ACAGCTTTTACATCTATGGTAATATTTTTGAAGTTAAATAGTATTAATTTTGAATGCACTCAAGATGAAGCGGTTCAGTT
TACTTTAAGAGTAGTAAACGACAAAAATCTTACTCTTGAGGGAATTGAGACATGGATCAAGAGGCATTGTAAATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14454.26 Da
• Isoelectric Point: 5.5653

>AT165451 WP_115259201.1 NZ_CP057506:c1138587-1138213 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSNYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT165451 NZ_CP073881:822159-822389 [Staphylococcus epidermidis]
ATGATTACTACAAGAAAACTAAGAAAAGCTGGTAATAGTTCAGTTGTTTCTGTACCGACAGAAGTGATCGCAGCACTCGG
AATATCAAATGGTGATAATCTTAAATTTAATGTAAAAGATAATAAAGTAACCATCGAAAAGGAGGTAAGAGAGGACGAGG
AGTTTTTTAAATTGTTGGATGAAACATTCACTGAGTATAACCAAGCTTTAAAAAGGATGGTTGACTTATAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2