Detailed information of TA system TA165450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 531288..531804
• Replicon: chromosome
• Accession: NZ_CP057506
• Organism: Citrobacter freundii strain RHB24-C05

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A5I6CHB9
• Locus tag: HVY06_RS02675
• Protein ID: WP_000220581.1
• Coordinates: 531520..531804 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HVY06_RS02670
• Protein ID: WP_115259576.1
• Coordinates: 531288..531530 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY06_RS02650	526303..527436	+	1134	WP_181819919.1	amidohydrolase/deacetylase family metallohydrolase	-
HVY06_RS02655	527420..528538	+	1119	WP_096758517.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
HVY06_RS02660	528535..529275	+	741	WP_115259578.1	KDGP aldolase family protein	-
HVY06_RS02665	529297..531210	+	1914	WP_181819920.1	BglG family transcription antiterminator	-
HVY06_RS02670	531288..531530	+	243	WP_115259576.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HVY06_RS02675	531520..531804	+	285	WP_000220581.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVY06_RS02680	531808..532272	-	465	WP_096759476.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
HVY06_RS02685	532394..534532	-	2139	WP_115259574.1	anaerobic ribonucleoside-triphosphate reductase	-
HVY06_RS02690	534951..536603	-	1653	WP_137400481.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10868.70 Da
• Isoelectric Point: 10.0482

>T165450 WP_000220581.1 NZ_CP057506:531520-531804 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQLKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVIVFVVAVGK
REHSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T165450 NZ_CP073881:693129-693491 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8947.32 Da
• Isoelectric Point: 5.0490

>AT165450 WP_115259576.1 NZ_CP057506:531288-531530 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLDKLNITPTEAVRLLFQYVAETGRMPVKTVTLSDSEDALIQTVRERLSSPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT165450 NZ_CP073881:692962-693132 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTTCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A5I6CHB9