Detailed information of TA system TA165390

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 297709..298295
• Replicon: chromosome
• Accession: NZ_CP057483
• Organism: Citrobacter sp. RHB25-C09

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HVY19_RS01335
• Protein ID: WP_181682648.1
• Coordinates: 297927..298295 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: HVY19_RS01330
• Protein ID: WP_181682647.1
• Coordinates: 297709..297930 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY19_RS01305	292716..293825	+	1110	WP_181682642.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
HVY19_RS01310	293873..294799	+	927	WP_181682643.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HVY19_RS01315	294796..296073	+	1278	WP_181682644.1	branched chain amino acid ABC transporter permease LivM	-
HVY19_RS01320	296070..296837	+	768	WP_181682645.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
HVY19_RS01325	296855..297568	+	714	WP_181682646.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
HVY19_RS01330	297709..297930	+	222	WP_181682647.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HVY19_RS01335	297927..298295	+	369	WP_181682648.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HVY19_RS01340	298495..299811	+	1317	WP_181682649.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
HVY19_RS01345	299918..300805	+	888	WP_181682650.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
HVY19_RS01350	300802..301647	+	846	WP_181682651.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
HVY19_RS01355	301649..302719	+	1071	WP_181682652.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	294796..303462	8666

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (28-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-52)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13681.97 Da
• Isoelectric Point: 7.3180

>T165390 WP_181682648.1 NZ_CP057483:297927-298295 [Citrobacter sp. RHB25-C09]
MTLQFISAEEIVRFHDKLLSVTPGVPGMPDPGRAEALLYRVLNKYEYEGIADVWILAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLKANPDFVEMTVEAAAGRLSLEEIVQRLRS

Nucleotide

• Length: 369 bp

>T165390 NZ_CP073850:693222-693584 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8043.01 Da
• Isoelectric Point: 5.6663

>AT165390 WP_181682647.1 NZ_CP057483:297709-297930 [Citrobacter sp. RHB25-C09]
MRTMNYSEARQNLASALESASTGTPVTITRRGHKSAVIISAEEFERYQAAKLDAEFDAIMGIHGSEIRELADK

Nucleotide

• Length: 222 bp

>AT165390 NZ_CP073850:693055-693225 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTTCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA