Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3571085..3571739 Replicon chromosome
Accession NZ_CP057481
Organism Escherichia fergusonii strain RHB25-C10

Toxin (Protein)


Gene name cptA Uniprot ID F0JPE9
Locus tag HVY20_RS17165 Protein ID WP_000244774.1
Coordinates 3571085..3571492 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HVY20_RS17170 Protein ID WP_000354046.1
Coordinates 3571473..3571739 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY20_RS17145 3567042..3568775 - 1734 WP_000813234.1 single-stranded-DNA-specific exonuclease RecJ -
HVY20_RS17150 3568781..3569491 - 711 WP_046076902.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HVY20_RS17155 3569516..3570412 - 897 WP_000806645.1 site-specific tyrosine recombinase XerD -
HVY20_RS17160 3570524..3571045 + 522 WP_001055874.1 flavodoxin FldB -
HVY20_RS17165 3571085..3571492 - 408 WP_000244774.1 protein YgfX Toxin
HVY20_RS17170 3571473..3571739 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HVY20_RS17175 3571991..3572971 + 981 WP_181665658.1 tRNA-modifying protein YgfZ -
HVY20_RS17180 3572992..3574296 + 1305 WP_181665659.1 hypothetical protein -
HVY20_RS17185 3574293..3574586 + 294 WP_181665660.1 hypothetical protein -
HVY20_RS17190 3574791..3575450 - 660 WP_000250283.1 hemolysin III family protein -
HVY20_RS17195 3575614..3575925 - 312 WP_001182952.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16078.05 Da        Isoelectric Point: 11.2511

>T165382 WP_000244774.1 NZ_CP057481:c3571492-3571085 [Escherichia fergusonii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T165382 NZ_CP073844:1024835-1025014 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT165382 WP_000354046.1 NZ_CP057481:c3571739-3571473 [Escherichia fergusonii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT165382 NZ_CP073844:1024212-1024811 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAGTTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L6ZX35

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References