Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3851973..3852556 Replicon chromosome
Accession NZ_CP057463
Organism Escherichia fergusonii strain RHB26-C03

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag HVY27_RS18905 Protein ID WP_000254750.1
Coordinates 3852221..3852556 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag HVY27_RS18900 Protein ID WP_000581937.1
Coordinates 3851973..3852221 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY27_RS18890 3848312..3849610 + 1299 WP_181198773.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
HVY27_RS18895 3849661..3851895 + 2235 WP_000226844.1 GTP diphosphokinase -
HVY27_RS18900 3851973..3852221 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HVY27_RS18905 3852221..3852556 + 336 WP_000254750.1 endoribonuclease MazF Toxin
HVY27_RS18910 3852627..3853418 + 792 WP_001071671.1 nucleoside triphosphate pyrophosphohydrolase -
HVY27_RS18915 3853646..3855283 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HVY27_RS18920 3855371..3856669 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T165352 WP_000254750.1 NZ_CP057463:3852221-3852556 [Escherichia fergusonii]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T165352 NZ_CP073827:2247734-2247922 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT165352 WP_000581937.1 NZ_CP057463:3851973-3852221 [Escherichia fergusonii]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT165352 NZ_CP073827:2247452-2247709 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References