Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2156409..2156985 Replicon chromosome
Accession NZ_CP057463
Organism Escherichia fergusonii strain RHB26-C03

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag HVY27_RS10710 Protein ID WP_001350372.1
Coordinates 2156698..2156985 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HVY27_RS10705 Protein ID WP_000063156.1
Coordinates 2156409..2156711 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY27_RS10685 2152223..2152735 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
HVY27_RS10690 2153005..2155155 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
HVY27_RS10695 2155205..2155408 + 204 WP_000467859.1 YbdD/YjiX family protein -
HVY27_RS10700 2155419..2156375 + 957 WP_001314406.1 GTPase -
HVY27_RS10705 2156409..2156711 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HVY27_RS10710 2156698..2156985 - 288 WP_001350372.1 BrnT family toxin Toxin
HVY27_RS10715 2157182..2158348 + 1167 WP_000800829.1 restriction endonuclease -
HVY27_RS10720 2158412..2160031 + 1620 WP_181199331.1 type I restriction-modification system subunit M -
HVY27_RS10725 2160021..2161328 + 1308 WP_181199332.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T165343 WP_001350372.1 NZ_CP057463:c2156985-2156698 [Escherichia fergusonii]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T165343 NZ_CP073824:c465029-464841 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT165343 WP_000063156.1 NZ_CP057463:c2156711-2156409 [Escherichia fergusonii]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT165343 NZ_CP073824:c465311-465054 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References