Detailed information of TA system TA165328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 165074..165984
• Replicon: plasmid pRHB26-C08_2
• Accession: NZ_CP057460
• Organism: Klebsiella pneumoniae strain RHB26-C08

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HVY29_RS26930
• Protein ID: WP_004883378.1
• Coordinates: 165074..165544 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: HVY29_RS26935
• Protein ID: WP_032413443.1
• Coordinates: 165541..165984 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY29_RS26900	160646..160813	-	168	WP_181672729.1	hypothetical protein	-
HVY29_RS26905	161160..161942	-	783	WP_181672730.1	IS21-like element ISSen3 family helper ATPase IstB	-
HVY29_RS26910	161939..162961	-	1023	WP_181672731.1	IS21-like element ISSen3 family transposase	-
HVY29_RS26915	163049..163374	+	326	Protein_170	transposase	-
HVY29_RS26920	163970..164149	-	180	WP_007372130.1	hypothetical protein	-
HVY29_RS26925	164228..164925	+	698	Protein_172	IS1 family transposase	-
HVY29_RS26930	165074..165544	-	471	WP_004883378.1	RES family NAD+ phosphorylase	Toxin
HVY29_RS26935	165541..165984	-	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
HVY29_RS26940	166085..166314	-	230	Protein_175	DUF4113 domain-containing protein	-
HVY29_RS26945	166355..166672	+	318	Protein_176	transposase	-
HVY29_RS26950	166886..167305	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	-
HVY29_RS26955	167302..167613	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	-
HVY29_RS26960	167818..168833	-	1016	Protein_179	IS3 family transposase	-
HVY29_RS26965	168892..170484	-	1593	WP_004902302.1	IS66-like element ISKox1 family transposase	-
HVY29_RS26970	170515..170865	-	351	WP_004189161.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..256547	256547
	-	inside	IScluster/Tn	-	-	161160..174439	13279

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17500.82 Da
• Isoelectric Point: 4.6155

>T165328 WP_004883378.1 NZ_CP057460:c165544-165074 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVLKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T165328 NZ_CP073808:c1996063-1995713 [Macrococcus canis]
ATGAAACGTGGAGATGTTTATTTAGCTGATCTGTCACCAGTGAAGGGGTCTGAACAAGGCGGTAAACGCCCCGTCGTTAT
TATTCAGAATAATATTGGGAATAAACATAGTCCTACAGTGATTGTAGCCGCAATTACAGCAAAGATTAATAAGGCAAAGA
TCCCCACCCATGTGGAAATTGGCAAGACCACGCATCACCTGGAAAAGGATTCGGTAATATTGCTTGAACAAATTAGAACT
ATTGATAAGAATAGGTTAATTGATCATCTGACAGTGCTTGATAAGGCAAAGATGAAAGAAGTGGATGTGGCACTTTTGAT
TAGTTTAGGGTTATCAATCGATAGTGAATAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT165328 WP_032413443.1 NZ_CP057460:c165984-165541 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT165328 NZ_CP073808:c1996204-1996064 [Macrococcus canis]
ATGAGCCAGAAGCTGGAGCAAATGCTGATAGAAGGATATCAATCAATGGCAGAGATTAATTTATCGATAGCATCAGAACA
TTATATATTAGAATGTGAAGTTAATGATAACCATCAGACATATACCCTTGATGGTGAATAG

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4