Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5296739..5297364 Replicon chromosome
Accession NZ_CP057459
Organism Klebsiella pneumoniae strain RHB26-C08

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HVY29_RS25700 Protein ID WP_002882817.1
Coordinates 5296739..5297122 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HVY29_RS25705 Protein ID WP_004150355.1
Coordinates 5297122..5297364 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY29_RS25685 5294105..5295007 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HVY29_RS25690 5295004..5295639 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HVY29_RS25695 5295636..5296565 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HVY29_RS25700 5296739..5297122 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HVY29_RS25705 5297122..5297364 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HVY29_RS25710 5297569..5298486 + 918 WP_009484979.1 alpha/beta hydrolase -
HVY29_RS25715 5298500..5299441 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HVY29_RS25720 5299486..5299923 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HVY29_RS25725 5299920..5300780 - 861 WP_004146232.1 virulence factor BrkB family protein -
HVY29_RS25730 5300774..5301373 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T165327 WP_002882817.1 NZ_CP057459:c5297122-5296739 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T165327 NZ_CP073808:1172882-1173118 [Macrococcus canis]
GTGATTAAACAATTACGAAAAATGGATGCCCAATCCAGTAGATTACTATTACAATGGATTAATAAAAATATTGTTCATTG
TGAAGATCCCAGAAGCTCAGGAAAAGCTTTGACTGGTAACTATAGTGGTTTTTGGCGTTATAGAGTTGGACAATTTCGTA
TTATTACTAAAATAGAAGATGATAAATTAATTATTTTAGTAATTTCAATAGATCATAGAAAAAATATTTATCGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT165327 WP_004150355.1 NZ_CP057459:c5297364-5297122 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT165327 NZ_CP073808:1172631-1172855 [Macrococcus canis]
ATGGCTACTATTACTATCAGGGTAAGCGAAGATGAAAAGAATTTTTTAAAATATATGTCAGAATTTATGAACTTAAGCTT
AACTGACTTAATTAAGAATTATACCATTGAAGATCTTGAAGATTTATTTGATACTCATGTTGCCGATAAAGCATATATTG
AATGGTCTAAAAATAAGCAGACTGTTTCTCACAATGATGTTATGCAAGAATTTGGATTACTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References