Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 84674..86297 Replicon plasmid pRHB28-C21_2
Accession NZ_CP057358
Organism Escherichia fergusonii strain RHB28-C21

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag HVY57_RS22845 Protein ID WP_000088046.1
Coordinates 84972..86297 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag HVY57_RS22840 Protein ID WP_024136327.1
Coordinates 84674..84967 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY57_RS22815 80546..81787 + 1242 WP_001053338.1 VWA domain-containing protein -
HVY57_RS22820 82350..82631 - 282 WP_000077926.1 hypothetical protein -
HVY57_RS22825 82681..82872 - 192 WP_000398480.1 hypothetical protein -
HVY57_RS22830 82964..83305 - 342 WP_001151575.1 hypothetical protein -
HVY57_RS22835 83678..84070 + 393 WP_000341066.1 hypothetical protein -
HVY57_RS22840 84674..84967 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
HVY57_RS22845 84972..86297 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
HVY57_RS22850 86358..86564 + 207 WP_000134171.1 hypothetical protein -
HVY57_RS22855 86666..86956 + 291 WP_000985910.1 hypothetical protein -
HVY57_RS22860 86998..87834 - 837 WP_000480968.1 aminoglycoside O-phosphotransferase APH(6)-Id -
HVY57_RS22865 87834..88637 - 804 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -
HVY57_RS22870 88923..90131 + 1209 WP_001339175.1 IS4-like element ISVsa5 family transposase -
HVY57_RS22875 90147..90266 - 120 Protein_87 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / aph(3'')-Ib / tet(B) / blaTEM-1B / aph(3')-Ia / aac(3)-IVa / aph(4)-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / tet(A) / dfrA12 / qacE / sul1 / aph(3')-IIa - 1..285327 285327


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T165284 WP_000088046.1 NZ_CP057358:84972-86297 [Escherichia fergusonii]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T165284 NZ_CP073788:c4723870-4723586 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT165284 WP_024136327.1 NZ_CP057358:84674-84967 [Escherichia fergusonii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT165284 NZ_CP073788:c4724102-4723860 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References