Detailed information of TA system TA165268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1668328..1668947
• Replicon: chromosome
• Accession: NZ_CP057357
• Organism: Escherichia fergusonii strain RHB28-C21

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: HVY57_RS08180
• Protein ID: WP_001280991.1
• Coordinates: 1668729..1668947 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HVY57_RS08175
• Protein ID: WP_104917851.1
• Coordinates: 1668328..1668702 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY57_RS08165	1663460..1664653	+	1194	WP_002431407.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HVY57_RS08170	1664676..1667825	+	3150	WP_001132493.1	multidrug efflux RND transporter permease subunit	-
HVY57_RS08175	1668328..1668702	+	375	WP_104917851.1	Hha toxicity modulator TomB	Antitoxin
HVY57_RS08180	1668729..1668947	+	219	WP_001280991.1	hemolysin expression modulator Hha	Toxin
HVY57_RS08185	1669444..1669914	+	471	WP_002431406.1	YlaC family protein	-
HVY57_RS08190	1670053..1671603	+	1551	WP_104917852.1	EAL domain-containing protein	-
HVY57_RS08195	1671645..1671998	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
HVY57_RS08205	1672377..1672688	+	312	WP_000409911.1	MGMT family protein	-
HVY57_RS08210	1672719..1673291	-	573	WP_000779831.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T165268 WP_001280991.1 NZ_CP057357:1668729-1668947 [Escherichia fergusonii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T165268 NZ_CP073783:c3800270-3799953 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14511.31 Da
• Isoelectric Point: 4.8989

>AT165268 WP_104917851.1 NZ_CP057357:1668328-1668702 [Escherichia fergusonii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT165268 NZ_CP073783:c3799960-3799673 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• PDB: 4ICG