Detailed information of TA system TA165253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4351433..4351968
• Replicon: chromosome
• Accession: NZ_CP057353
• Organism: Citrobacter freundii strain RHB29-C07

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HVY60_RS20635
• Protein ID: WP_137399199.1
• Coordinates: 4351681..4351968 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D4BKM9
• Locus tag: HVY60_RS20630
• Protein ID: WP_006688247.1
• Coordinates: 4351433..4351684 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY60_RS20600	4346445..4347293	+	849	WP_135912711.1	alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-lyase PhnJ	-
HVY60_RS20605	4347290..4348048	+	759	WP_115259672.1	phosphonate C-P lyase system protein PhnK	-
HVY60_RS20610	4348157..4348837	+	681	WP_117343579.1	phosphonate C-P lyase system protein PhnL	-
HVY60_RS20615	4348834..4349970	+	1137	WP_096758663.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
HVY60_RS20620	4349973..4350518	+	546	WP_096758664.1	ribose 1,5-bisphosphokinase	-
HVY60_RS20625	4350591..4351349	+	759	WP_181695088.1	phosphonate metabolism protein PhnP	-
HVY60_RS20630	4351433..4351684	+	252	WP_006688247.1	plasmid stabilization protein	Antitoxin
HVY60_RS20635	4351681..4351968	+	288	WP_137399199.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVY60_RS20640	4351965..4352294	-	330	WP_096758667.1	hypothetical protein	-
HVY60_RS20645	4352363..4354627	-	2265	WP_181695089.1	hybrid sensor histidine kinase/response regulator	-
HVY60_RS20650	4354742..4356265	+	1524	WP_181695090.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11173.02 Da
• Isoelectric Point: 10.2830

>T165253 WP_137399199.1 NZ_CP057353:4351681-4351968 [Citrobacter freundii]
MSYTVKFREDALKEWQKLDKTIQQQFAKELKKCGENPHVPSAKLRGIKDCYKIQLRTSRFRLVYQVIDDTLVIAVVAVGK
RERSEVYNLASERLR

Nucleotide

• Length: 288 bp

>T165253 NZ_CP073782:2837945-2838313 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9215.53 Da
• Isoelectric Point: 4.2965

>AT165253 WP_006688247.1 NZ_CP057353:4351433-4351684 [Citrobacter freundii]
MPHIILSDTTASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQQLVKLVHERSQQPLVDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT165253 NZ_CP073782:2837727-2837948 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7X1BT32