Detailed information of TA system TA165239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 246959..247545
• Replicon: chromosome
• Accession: NZ_CP057353
• Organism: Citrobacter freundii strain RHB29-C07

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HVY60_RS01190
• Protein ID: WP_181638419.1
• Coordinates: 247177..247545 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: HVY60_RS01185
• Protein ID: WP_096759232.1
• Coordinates: 246959..247180 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY60_RS01160	242016..243125	+	1110	WP_115257271.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
HVY60_RS01165	243173..244099	+	927	WP_096759229.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HVY60_RS01170	244096..245376	+	1281	WP_096759230.1	branched chain amino acid ABC transporter permease LivM	-
HVY60_RS01175	245373..246140	+	768	WP_096759231.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
HVY60_RS01180	246158..246871	+	714	WP_096759502.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
HVY60_RS01185	246959..247180	+	222	WP_096759232.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HVY60_RS01190	247177..247545	+	369	WP_181638419.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HVY60_RS01195	247799..249115	+	1317	WP_096759233.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
HVY60_RS01200	249220..250107	+	888	WP_096759234.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
HVY60_RS01205	250104..250949	+	846	WP_115257274.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
HVY60_RS01210	250951..252021	+	1071	WP_115257275.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	242016..252761	10745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13708.98 Da
• Isoelectric Point: 6.7255

>T165239 WP_181638419.1 NZ_CP057353:247177-247545 [Citrobacter freundii]
MTLQIISAEEIIQFHDRLLRVTPGVTGMPDPGRAEALMYRVLNQIEYEDVTDVWLLAAMHLLAISRGYIFNDGNKRTALF
ITLLFLKRNGIALSANPDFVAMTVEAAAGRLSLEQIAHRLRQ

Nucleotide

• Length: 369 bp

>T165239 NZ_CP073771:c2295700-2295215 [Enterobacter hormaechei]
GTGGGGCGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTGTACTCACTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATACCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCCGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8260.32 Da
• Isoelectric Point: 4.7769

>AT165239 WP_096759232.1 NZ_CP057353:246959-247180 [Citrobacter freundii]
MRTVNYSEARQNLAEVLDSAVTGVPVTITRRGHKPAVIISAEEFERYQAARLDDEFADIMMIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT165239 NZ_CP073771:c2295954-2295688 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACAGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATC
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAGAAACTGCTT
GCAAGGAAACCGCAGTGGGGCGTGTAA