Detailed information of TA system TA165236

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 5323033..5323736
• Replicon: chromosome
• Accession: NZ_CP057330
• Organism: Klebsiella oxytoca strain RHB30-C02

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: HVY70_RS25000
• Protein ID: WP_049017155.1
• Coordinates: 5323033..5323374 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HVY70_RS25005
• Protein ID: WP_049243960.1
• Coordinates: 5323395..5323736 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY70_RS24975	5318628..5319422	+	795	WP_064345377.1	DNA/RNA non-specific endonuclease	-
HVY70_RS24980	5319712..5320035	+	324	WP_064345376.1	endoribonuclease SymE	-
HVY70_RS24985	5320194..5320628	+	435	WP_004117617.1	VOC family protein	-
HVY70_RS24990	5320794..5321537	+	744	WP_064345375.1	hypothetical protein	-
HVY70_RS24995	5321767..5322774	-	1008	WP_049017154.1	restriction endonuclease	-
HVY70_RS25000	5323033..5323374	-	342	WP_049017155.1	TA system toxin CbtA family protein	Toxin
HVY70_RS25005	5323395..5323736	-	342	WP_049243960.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HVY70_RS25010	5323747..5324289	-	543	WP_064345374.1	DNA repair protein RadC	-
HVY70_RS25015	5324302..5324745	-	444	WP_064345373.1	antirestriction protein	-
HVY70_RS25020	5324776..5325597	-	822	WP_064345372.1	DUF945 domain-containing protein	-
HVY70_RS25025	5325718..5326191	-	474	WP_064345371.1	hypothetical protein	-
HVY70_RS25030	5326263..5326715	-	453	WP_032742543.1	hypothetical protein	-
HVY70_RS25035	5326751..5327467	-	717	WP_073970829.1	WYL domain-containing protein	-
HVY70_RS25040	5327711..5328586	-	876	WP_064345369.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5319712..5339989	20277

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12724.66 Da
• Isoelectric Point: 6.4514

>T165236 WP_049017155.1 NZ_CP057330:c5323374-5323033 [Klebsiella oxytoca]
MKTLPATTPQAATLCLSPVAVWQMLLACLLEQHYGLTLNDTPFSDETVIQEHIDAGITLADAVNFLVEKYELVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAQ

Nucleotide

• Length: 342 bp

>T165236 NZ_CP073771:579935-580309 [Enterobacter hormaechei]
ATGCAAATACAATCCCTGCCCCCTCAAAGGGCGGCATCGTCACGTCTGTCATCAGTCGAAATCTGGCAAAAATTACTGGA
GTATTTGCTGGAGCAACACTACGGACTTACCCTCAACGACACGCCGTTTGGCAACGACAGTGTGATTCAGAAACACATTG
ATGCGGGAATCTCACTGTGTGACGCGGTAAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTC
AGCGTGAAAGAGCAGTCTCCTTTTATCGGCAGCATTGATATGCTCCGCGCCCGGAAGGCTACCGGGCTGATGACCCGCAA
GGGATATAAAACCGTTACTGACATCACAGGCGGCAGATTCAGCGAAGGGAAATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12840.57 Da
• Isoelectric Point: 6.7344

>AT165236 WP_049243960.1 NZ_CP057330:c5323736-5323395 [Klebsiella oxytoca]
MKYTDSENDSFLKWGLNRNVTPCFVARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTHR

Nucleotide

• Length: 342 bp

>AT165236 NZ_CP073771:579538-579897 [Enterobacter hormaechei]
ATGCAGAAAGCAACCCGTGTAATCAACCATAACATCGCGGAACCCTGGTGGGGCCTCAGACGCAACGTCACGCCATGTTT
TGGTGCCCGGCTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCTGACCGCGCCAGCACCACCGGGACATTTAATGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGTTACTGATGAAGCAGATGGAGCTCATGCTGACCAGCAGCGAACTCATG
CCCCGCATTCAGCGCTGCATCACGCTCCATGCAAAAGGGCTGATATGTGAGGCCGATACGCTCGGTTCCTGTGGCTATTT
GTACATCGTAATTTATCCGGCGTCTGCAACAACCGCATAA