Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 63553..64130 Replicon plasmid pRHB30-C05_3
Accession NZ_CP057315
Organism Klebsiella pneumoniae strain RHB30-C05

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G3NNX6
Locus tag HVY73_RS26210 Protein ID WP_059065587.1
Coordinates 63798..64130 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7G3NP82
Locus tag HVY73_RS26205 Protein ID WP_032454911.1
Coordinates 63553..63798 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY73_RS26170 59281..60128 - 848 Protein_58 IS3 family transposase -
HVY73_RS26175 60201..60518 - 318 Protein_59 helix-turn-helix domain-containing protein -
HVY73_RS26180 60732..61112 + 381 Protein_60 transposase family protein -
HVY73_RS26185 61090..61308 + 219 WP_176455421.1 hypothetical protein -
HVY73_RS26190 61318..61575 + 258 WP_060655703.1 cloacin immunity family protein -
HVY73_RS26195 61772..62464 - 693 WP_094313752.1 Exc2 family lipoprotein -
HVY73_RS26200 62513..62848 - 336 WP_170933330.1 TonB family protein -
HVY73_RS26205 63553..63798 + 246 WP_032454911.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HVY73_RS26210 63798..64130 + 333 WP_059065587.1 endoribonuclease MazF Toxin
HVY73_RS26215 64195..64287 + 93 Protein_67 IS3 family transposase -
HVY73_RS26220 64724..66187 - 1464 Protein_68 group II intron reverse transcriptase/maturase -
HVY73_RS26225 66176..67363 - 1188 Protein_69 ISL3 family transposase -
HVY73_RS26230 67481..67930 - 450 WP_105910642.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iutA / iucD / iucC / iucB / iucA / cseA 1..147224 147224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11991.87 Da        Isoelectric Point: 7.7494

>T165222 WP_059065587.1 NZ_CP057315:63798-64130 [Klebsiella pneumoniae]
MVSRFVPDAGDLIWINFDPVEGHEQGGHRPAVVLSPFAYNNKTGLLLCVPCTTKVKGYPFEVELPGERDGVALADQITCV
DWRARKVEKKGKVATGELSEIRAKAKALIG

Download         Length: 333 bp

>T165222 NZ_CP073768:2377849-2379171 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATTACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGTGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8881.21 Da        Isoelectric Point: 5.7996

>AT165222 WP_032454911.1 NZ_CP057315:63553-63798 [Klebsiella pneumoniae]
MSQATFKKWGNQAGIRFPAAVMKAAALNVDDTVDIEVQEGRIVLIPTKEKTYSLDALLSGITEENMHNKADFGKPTGKEM
L

Download         Length: 246 bp

>AT165222 NZ_CP073768:2377583-2377849 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G3NNX6

Antitoxin

Source ID Structure
AlphaFold DB A0A7G3NP82

References