Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 52613..53256 Replicon plasmid pRHB30-C05_3
Accession NZ_CP057315
Organism Klebsiella pneumoniae strain RHB30-C05

Toxin (Protein)


Gene name vagD Uniprot ID A0A6H2GRP8
Locus tag HVY73_RS26115 Protein ID WP_023292131.1
Coordinates 52840..53256 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A6B7Q5S4
Locus tag HVY73_RS26110 Protein ID WP_021312476.1
Coordinates 52613..52843 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY73_RS26085 47680..48857 + 1178 Protein_41 IS3 family transposase -
HVY73_RS26090 49239..50024 - 786 WP_050485696.1 site-specific integrase -
HVY73_RS26095 50070..50591 - 522 WP_094313782.1 hypothetical protein -
HVY73_RS26100 50649..51041 - 393 WP_170933338.1 hypothetical protein -
HVY73_RS26105 51078..52022 - 945 WP_094313783.1 hypothetical protein -
HVY73_RS26110 52613..52843 + 231 WP_021312476.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HVY73_RS26115 52840..53256 + 417 WP_023292131.1 type II toxin-antitoxin system VapC family toxin Toxin
HVY73_RS26120 53291..53536 + 246 WP_065801591.1 hypothetical protein -
HVY73_RS26125 53600..53854 + 255 WP_095153353.1 hypothetical protein -
HVY73_RS26130 54411..55417 + 1007 Protein_50 IS110 family transposase -
HVY73_RS26135 55696..55944 + 249 WP_065801593.1 hypothetical protein -
HVY73_RS26140 55949..56521 + 573 WP_094309628.1 hypothetical protein -
HVY73_RS26145 56552..57046 + 495 WP_094313750.1 DNA-binding protein -
HVY73_RS26150 57107..57310 + 204 WP_004150739.1 hemolysin expression modulator Hha -
HVY73_RS26155 57359..57568 + 210 WP_094308932.1 hypothetical protein -
HVY73_RS26160 57598..57906 - 309 Protein_56 DDE-type integrase/transposase/recombinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iutA / iucD / iucC / iucB / iucA / cseA 1..147224 147224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14975.40 Da        Isoelectric Point: 8.5464

>T165221 WP_023292131.1 NZ_CP057315:52840-53256 [Klebsiella pneumoniae]
VKKTFMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN

Download         Length: 417 bp

>T165221 NZ_CP073768:2058380-2058483 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8723.76 Da        Isoelectric Point: 4.3410

>AT165221 WP_021312476.1 NZ_CP057315:52613-52843 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Download         Length: 231 bp

>AT165221 NZ_CP073768:c2058521-2058250 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6H2GRP8

Antitoxin

Source ID Structure
AlphaFold DB A0A6B7Q5S4

References