Detailed information of TA system TA165220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 34235..35145
• Replicon: plasmid pRHB30-C05_2
• Accession: NZ_CP057314
• Organism: Klebsiella pneumoniae strain RHB30-C05

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HVY73_RS25100
• Protein ID: WP_004883378.1
• Coordinates: 34675..35145 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: HVY73_RS25095
• Protein ID: WP_004026357.1
• Coordinates: 34235..34678 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY73_RS25075	31301..31516	-	216	Protein_41	IS200/IS605 family transposase	-
HVY73_RS25080	31518..32087	+	570	Protein_42	IS200/IS605 family element transposase accessory protein TnpB	-
HVY73_RS25085	32442..32864	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
HVY73_RS25090	32864..34135	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
HVY73_RS25095	34235..34678	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
HVY73_RS25100	34675..35145	+	471	WP_004883378.1	RES family NAD+ phosphorylase	Toxin
HVY73_RS25105	35254..35514	-	261	WP_004026352.1	hypothetical protein	-
HVY73_RS25110	36237..36395	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
HVY73_RS25115	37001..37975	+	975	WP_004883382.1	hypothetical protein	-
HVY73_RS25120	38173..38550	-	378	WP_004883386.1	hypothetical protein	-
HVY73_RS25125	38781..39077	+	297	WP_094284716.1	hydrogenase expression/formation protein HypD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..194082	194082
	-	flank	IS/Tn	-	-	31734..32087	353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17500.82 Da
• Isoelectric Point: 4.6155

>T165220 WP_004883378.1 NZ_CP057314:34675-35145 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVLKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T165220 NZ_CP073768:c1893299-1892925 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT165220 WP_004026357.1 NZ_CP057314:34235-34678 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT165220 NZ_CP073768:c1893756-1893388 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A6P1V3Q9