Detailed information of TA system TA165219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5068559..5069184
• Replicon: chromosome
• Accession: NZ_CP057313
• Organism: Klebsiella pneumoniae strain RHB30-C05

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: HVY73_RS24505
• Protein ID: WP_002882817.1
• Coordinates: 5068559..5068942 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HVY73_RS24510
• Protein ID: WP_074185945.1
• Coordinates: 5068942..5069184 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY73_RS24490	5065925..5066827	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
HVY73_RS24495	5066824..5067459	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
HVY73_RS24500	5067456..5068385	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
HVY73_RS24505	5068559..5068942	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
HVY73_RS24510	5068942..5069184	-	243	WP_074185945.1	ribbon-helix-helix domain-containing protein	Antitoxin
HVY73_RS24515	5069389..5070306	+	918	WP_040223557.1	alpha/beta hydrolase	-
HVY73_RS24520	5070320..5071261	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
HVY73_RS24525	5071306..5071743	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HVY73_RS24530	5071740..5072600	-	861	WP_004146232.1	virulence factor BrkB family protein	-
HVY73_RS24535	5072594..5073193	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T165219 WP_002882817.1 NZ_CP057313:c5068942-5068559 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T165219 NZ_CP073768:1020030-1020365 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9537.74 Da
• Isoelectric Point: 4.3509

>AT165219 WP_074185945.1 NZ_CP057313:c5069184-5068942 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGTEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT165219 NZ_CP073768:1019782-1020030 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8