Detailed information of TA system TA165205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1756129..1756719
• Replicon: chromosome
• Accession: NZ_CP057313
• Organism: Klebsiella pneumoniae strain RHB30-C05

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HVY73_RS08490
• Protein ID: WP_021466317.1
• Coordinates: 1756387..1756719 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: HVY73_RS08485
• Protein ID: WP_000288812.1
• Coordinates: 1756129..1756386 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY73_RS08470	1753472..1754251	-	780	WP_048291202.1	hypothetical protein	-
HVY73_RS08475	1755119..1755591	+	473	Protein_1656	hypothetical protein	-
HVY73_RS08480	1755588..1755794	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
HVY73_RS08485	1756129..1756386	+	258	WP_000288812.1	antitoxin	Antitoxin
HVY73_RS08490	1756387..1756719	+	333	WP_021466317.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HVY73_RS08500	1757042..1758478	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
HVY73_RS08510	1758844..1760298	-	1455	WP_004148975.1	AMP nucleosidase	-
HVY73_RS08515	1760428..1760673	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1746382..1756719	10337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11899.82 Da
• Isoelectric Point: 10.4722

>T165205 WP_021466317.1 NZ_CP057313:1756387-1756719 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDAVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T165205 NZ_CP073764:3113423-3113842 [Methylovirgula sp. HY1]
ATGATCTGCCTGGATACAAATGCGGTGATCGCGGTCATCAACGGTCGCACACCGGCGGTGCGCGTTCATCTGGAGGCCGC
GCTAAGCGCGGGCCAGCAAGTCGCTGTCCCCGTGATCGTTCTGTTCGAGCTCTGGTTCGGGATCGTTAAGAGCCTGAGAG
TGGCAGCGAATAAAAGGGCTCTCAGCGTATTTTTTGCTAATGGCGTTGAGATACTGCCGTTCGATGCCGACGATGCCCGC
GAAGCCGGCGACATTCGTGGCGTTCTTGCCAGGGCTGGCTCACCGATCGGCGCTTATGACATCCTCATCGCGGCGCAAGC
CAGGCGGCGTGACGCGCTTCTGGTCACCGCGAATGGCCGCGAATTTGTCCGTGTCCCGGGATTGAGGACGGAGGATTGGG
CGGCGCCATCCGGCCAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT165205 WP_000288812.1 NZ_CP057313:1756129-1756386 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT165205 NZ_CP073764:3113151-3113426 [Methylovirgula sp. HY1]
ATGAGCGATTCCGGGACCGCAAAAATTTTCATGCATGGACGCAGCCAAGCGGTGCGTCTGCCGAAAGAGTTTCGTCTTCC
AGGCCAGGAAGTACGGGTGACCCGGGTTGGACGCGGCGTCCTGCTGGAACCGATCGCAAGCGATGCAAAGGACATCCGGG
CGTGGTTCGCGGAACTCGATCGTTCGGGTGGGGCTGATTTTTTGCCGGAAGGGCGGCCGGAACAGCCGCCGATGCCTCCC
GAGGAAGTAATTTTCGAAGATGAACCGGGCAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0