Detailed information of TA system TA165204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1411572..1412308
• Replicon: chromosome
• Accession: NZ_CP057313
• Organism: Klebsiella pneumoniae strain RHB30-C05

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HVY73_RS06970
• Protein ID: WP_065809207.1
• Coordinates: 1411826..1412308 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HVY73_RS06965
• Protein ID: WP_003026799.1
• Coordinates: 1411572..1411838 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY73_RS06935	1407517..1409124	+	1608	WP_117099992.1	prolyl oligopeptidase family serine peptidase	-
HVY73_RS06940	1409521..1409784	+	264	WP_065809210.1	hypothetical protein	-
HVY73_RS06945	1409781..1410347	+	567	WP_134905762.1	hypothetical protein	-
HVY73_RS06950	1410378..1410872	+	495	WP_065809208.1	DNA-binding protein	-
HVY73_RS06955	1410932..1411134	+	203	Protein_1354	hemolysin expression modulator Hha	-
HVY73_RS06960	1411148..1411378	+	231	WP_004213598.1	hypothetical protein	-
HVY73_RS06965	1411572..1411838	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HVY73_RS06970	1411826..1412308	+	483	WP_065809207.1	GNAT family N-acetyltransferase	Toxin
HVY73_RS06975	1412669..1413268	-	600	WP_117100032.1	helix-turn-helix transcriptional regulator	-
HVY73_RS06980	1413482..1414426	-	945	WP_077254249.1	fimbrial protein	-
HVY73_RS06985	1414438..1415016	-	579	WP_032432061.1	type 1 fimbrial protein	-
HVY73_RS06990	1415020..1415760	-	741	WP_050597964.1	molecular chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1387578..1423252	35674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17365.94 Da
• Isoelectric Point: 9.1200

>T165204 WP_065809207.1 NZ_CP057313:1411826-1412308 [Klebsiella pneumoniae]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T165204 NZ_CP073764:c2735676-2735329 [Methylovirgula sp. HY1]
ATGAGACGGGGCGAAATATGGACCGTCGCCGGCGGCAAGGACTATGTCGGCAAACCTCGGCCAGCAGTCATCATACAAGA
CAATAGTTTCGACGCGACAGACTCCATCACTATCTGCGCCTTCACAACGGATGAAACCGACGCGCCGTTATTTCGTCTCC
CTGTGGGGGGCACCGACCGAAATGGCTTGAACAGCACGTGTCGGCTCATGGTCGACAAGATCACGACCGTGCCAAAATCC
AAGATCGGAACCTTTATCGGGCAGTTGGATGAAGAAGATATTTTGCGGCTCAATCAGGCCGTACTCGTTTTCCTTGGCCT
AGCAATGTCGCCAAAAGCGCGCCGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT165204 WP_003026799.1 NZ_CP057313:1411572-1411838 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT165204 NZ_CP073764:c2735897-2735673 [Methylovirgula sp. HY1]
ATGGTCTCTGTAAACAAAAGCAACAGCTCGCGGGACAAGGTGCGGACCCATCGCGAACGCCTGCGCGCCCAGGGTTTGCG
CCCGATCCAGATTTGGGTGCCCGACATGCGAGCGCCCGCATTCCGATCGGAAGCGCATAGGCAGTCATTAGCCGTCGCTC
AGAGCGCCCATGCCGCCGAGGATCAGAGCTTCATCGACGCCATAACGGATCGGAATAATGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66