Detailed information of TA system TA165201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 323642..324228
• Replicon: chromosome
• Accession: NZ_CP057313
• Organism: Klebsiella pneumoniae strain RHB30-C05

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HVY73_RS01505
• Protein ID: WP_021312828.1
• Coordinates: 323860..324228 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: HVY73_RS01500
• Protein ID: WP_004174006.1
• Coordinates: 323642..323863 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY73_RS01480	319799..320725	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HVY73_RS01485	320722..321999	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
HVY73_RS01490	321996..322763	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
HVY73_RS01495	322765..323478	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
HVY73_RS01500	323642..323863	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HVY73_RS01505	323860..324228	+	369	WP_021312828.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HVY73_RS01510	324501..325817	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
HVY73_RS01515	325924..326811	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
HVY73_RS01520	326808..327653	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
HVY73_RS01525	327655..328725	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	318628..329462	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13578.96 Da
• Isoelectric Point: 8.5493

>T165201 WP_021312828.1 NZ_CP057313:323860-324228 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGYIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T165201 NZ_CP073764:c26289-26020 [Methylovirgula sp. HY1]
TTGACGTGGCAGATCGAATTCGATGCGGCAGCCTTGGATGATTTACGCAAGCTCGGGAGCGCCGGCCGCGTCCACGTCCT
GCGGTTTCTTCAAGAGCGCATCGCGCCCGCGGATGATCCGCGCCGCCTCGGCAAAGCGTTGAGCGGCGACAAGCCGGGCT
TCTGGCGCTATCGTGTCGGCGACTATCGGATCATCGCCCGCATCGAAGACCAAAGACTCATGGTTCTCATCATCGCCATC
GGCCATCGTAGCGAAATCTACCGCAAATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT165201 WP_004174006.1 NZ_CP057313:323642-323863 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT165201 NZ_CP073764:c26504-26286 [Methylovirgula sp. HY1]
ATGCTCGCCCTACGTTTACCCCCCGACATCGAGAAGCGCCTCGACGAACTGGCGCGCAAGACCGGCCGCACGAAGAGCTA
TTATGCGCGCAAGGCGATCATCGAAATGATCGAAGATCTGAATGATCTCGAACTTGCCAAGGCCCGGCTTGCCGAAAACG
ATGAGCGCATCCCGCTTGAGCAGATTCTCGACGAGTTCGCGGCCGAGCTTAACGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7