Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 83338..84961 Replicon plasmid pRHB30-C07_2
Accession NZ_CP057312
Organism Escherichia fergusonii strain RHB30-C07

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag HVY75_RS22455 Protein ID WP_000088046.1
Coordinates 83636..84961 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag HVY75_RS22450 Protein ID WP_024136327.1
Coordinates 83338..83631 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY75_RS22425 79210..80451 + 1242 WP_001053338.1 VWA domain-containing protein -
HVY75_RS22430 81014..81295 - 282 WP_000077926.1 hypothetical protein -
HVY75_RS22435 81345..81536 - 192 WP_000398480.1 hypothetical protein -
HVY75_RS22440 81628..81969 - 342 WP_001151575.1 hypothetical protein -
HVY75_RS22445 82342..82734 + 393 WP_000341066.1 hypothetical protein -
HVY75_RS22450 83338..83631 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
HVY75_RS22455 83636..84961 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
HVY75_RS22460 85022..85228 + 207 WP_000134171.1 hypothetical protein -
HVY75_RS22465 85330..85620 + 291 WP_000985910.1 hypothetical protein -
HVY75_RS22470 85662..86498 - 837 WP_000480968.1 aminoglycoside O-phosphotransferase APH(6)-Id -
HVY75_RS22475 86498..87301 - 804 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -
HVY75_RS22480 87587..88795 + 1209 WP_001352368.1 IS4-like element ISVsa5 family transposase -
HVY75_RS22485 88811..88930 - 120 Protein_86 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / aph(3'')-Ib / tet(B) / blaTEM-1B / aph(3')-Ia / aac(3)-IVa / aph(4)-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / tet(A) / dfrA12 / qacE / sul1 / aph(3')-IIa - 1..277375 277375


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T165199 WP_000088046.1 NZ_CP057312:83636-84961 [Escherichia fergusonii]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T165199 NZ_CP073754:c3229871-3229572 [Methylomonas paludis]
ATGACTGAAAAAATTAGTGTTGCCAGTTTACCTGAGTTTGATGCATCACATTATTTGGATAGCGAACATGCAATTGCGGA
ATATTTAACAGTCATTATTGAAGACGGCGATTCAGCTTTGCTTGCCGCAGCGCTAGGGGATATTGCGCGTGCCCGAGGCA
TGGCGGATATTGCTAAAGCATCCGGCCTAACCCGTGAGGCGCTTTATAAAGCGCTAAGACCTAATGCTCATCCTCGTTTC
GATACAATCGCTAAAGTATGTAAGGCATTAGGCGTAAAATTGCAGGTTGAGCCTATTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT165199 WP_024136327.1 NZ_CP057312:83338-83631 [Escherichia fergusonii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT165199 NZ_CP073754:c3230171-3229875 [Methylomonas paludis]
ATGTACATTATTCGCACCCTTCCTCAGTTCGATAAATGGCTATCCGGCATTAAGGACGGCATGACCAAGCGTCGCCTGGT
AGCCAGAATTAAAAAAGCCCAGCTGGGCAATCTGGGCGACATCAAATCGGTTGGAGAAGGAGTTTTCGAAATGCGTGAAC
ATTTTGGTGCAGGTTACCGGATGTATTATCTACAGCATGGCGCAGTAATGATAATCATGTTGGGTGGTGGGGACAAATCA
TCACAAGCAGATGATATTGCAAAAGCCAAACAACTAGCCAATCTTTTGGAGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References