Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2122957..2123533 Replicon chromosome
Accession NZ_CP057311
Organism Escherichia fergusonii strain RHB30-C07

Toxin (Protein)


Gene name shpA Uniprot ID A0A7W3EF74
Locus tag HVY75_RS10420 Protein ID WP_024256534.1
Coordinates 2123246..2123533 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HVY75_RS10415 Protein ID WP_000063156.1
Coordinates 2122957..2123259 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY75_RS10395 2118771..2119283 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
HVY75_RS10400 2119553..2121703 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
HVY75_RS10405 2121753..2121956 + 204 WP_000467859.1 YbdD/YjiX family protein -
HVY75_RS10410 2121967..2122923 + 957 WP_001355122.1 GTPase -
HVY75_RS10415 2122957..2123259 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HVY75_RS10420 2123246..2123533 - 288 WP_024256534.1 BrnT family toxin Toxin
HVY75_RS10425 2123689..2124423 - 735 WP_000208601.1 Fic family protein -
HVY75_RS10430 2124686..2128198 + 3513 WP_181202857.1 type I restriction-modification system endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11241.74 Da        Isoelectric Point: 7.4020

>T165186 WP_024256534.1 NZ_CP057311:c2123533-2123246 [Escherichia fergusonii]
MPMEFEWDANKAKSNRMKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T165186 NZ_CP073745:c2154371-2154060 [Bacterioplanoides sp. SCSIO 12839]
ATGCATCTTATTTCCAGAAAGCCGTTTAACGACGCCGTGAAGAAATATCCCAATCAGGCTTCGGCCATAGAAGGGATCTA
TAAAACGCTGCGAAATGGAAACTTCCATGACCCCGCTGCGTTGCGCTCAGTGTTCCCAAGCCTAGACAACTTTAAGTACA
AAGATAAATGGTGGGTCATTGATATTGGTGGTAACAAACTGCGGCTGATCGCTTTTATCGAGTTCCGTGATAATCGCATG
TATGTGAAGCACATTGTGTCGCATGCGGAATACGACAAGTTGTGTAGAAAGTACGCGAAAGAAGCAGAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT165186 WP_000063156.1 NZ_CP057311:c2123259-2122957 [Escherichia fergusonii]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT165186 NZ_CP073745:c2154047-2153607 [Bacterioplanoides sp. SCSIO 12839]
ATGAGTTTCTCCAGTATTAAAACGATGGCTAGCGACCTGTTTAATCAAGCTGGCTTTGTCAGCAAAATCCATAATGACGA
CGAATACCAACAAGCATTGGCGTTAATGGATGAGCTGATTGAAGAGTATGACAACTACGTTCCTCTGATTGAAGTATTGG
CTGCTTCCATTGAAAAATGGGAAAACGAATCAGAAGAGTTCGCTGAGTTTAATCAGCGTATTAATGCGCTGGATGATGGC
GTAGCAGTGCTTCGCACTCTGATGGATCAGTATCAGTTAAAAGCTGATGACTTAAAAAATGAGATTGGCAGTAAAAGTTT
AGTATCCATGATTCTTAATGGCTCGCGCAAATTAACGCGTGATCATATTCAGGCACTTTCAGAGCGTTTTCATGTATCGC
CTGCGGTGTTTTTTAATATGGAGTCGGTGCGCGCTGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3EF74

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References