Detailed information of TA system TA165169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3551843..3552393
• Replicon: chromosome
• Accession: NZ_CP057266
• Organism: Escherichia fergusonii strain RHB31-C13

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HVY87_RS17260
• Protein ID: WP_032183189.1
• Coordinates: 3552079..3552393 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: HVY87_RS17255
• Protein ID: WP_000125566.1
• Coordinates: 3551843..3552076 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY87_RS17230	3546915..3547202	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
HVY87_RS17235	3547262..3548593	+	1332	WP_001183184.1	MFS transporter	-
HVY87_RS17240	3548700..3549230	+	531	WP_032183187.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
HVY87_RS17245	3549223..3551085	+	1863	WP_000377102.1	glutathione-regulated potassium-efflux system protein KefC	-
HVY87_RS17250	3551278..3551757	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
HVY87_RS17255	3551843..3552076	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HVY87_RS17260	3552079..3552393	+	315	WP_032183189.1	CcdB family protein	Toxin
HVY87_RS17265	3552390..3553238	-	849	WP_000257189.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
HVY87_RS17270	3553245..3553622	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
HVY87_RS17275	3553625..3554446	-	822	WP_001065375.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
HVY87_RS17280	3554443..3555432	-	990	WP_046083039.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
HVY87_RS17285	3555432..3556718	-	1287	WP_060765559.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11694.65 Da
• Isoelectric Point: 8.0666

>T165169 WP_032183189.1 NZ_CP057266:3552079-3552393 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLADGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T165169 NZ_CP073721:7035469-7035726 [Dactylosporangium roseum]
ATGACACAGGCGATCAGCGCCAGCGAGGCGCGGAAGAGCTTGTTTCCACTCATCGAGCAGGTCAACAACGACCATACGCC
GATCGAGATCGTATCTAAGCGGGGCAACGCGGTCCTGGTCAGCAAGGAAGACTGGGACGCGATTGTCGAAACCAACTACC
TGCTGCGCTCCCCCGCCAACGCAAAGCACTTGATGGCAAGCGTCGAGCAGTGGCGCTCGGGGCAGGCGACGGAACGGGAC
CTGGATTCCGACGCGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT165169 WP_000125566.1 NZ_CP057266:3551843-3552076 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT165169 NZ_CP073721:7035723-7035983 [Dactylosporangium roseum]
GTGAAGCTCAGTTGGACTGACCTCGCCTGGGACGACTATCTGTACTGGCAGACGCAGGACCGCAAGACGCTCAGGCGCAT
CAACGCCTTGATCGCAGACATCAAGCGCGATCCAGACGGACCTGGCATCGGTAAACCCGAGCTCCTGAGGAACAATCTGG
CCGGCCTACGCTCCCGCCGAATCGACGACGAGCACCGCCTCGTTTACGCCGTCACACCAGACGAAGTAACAATCATCTCC
TGCCGCTACCGCTACCAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9