Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1894148..1894802 Replicon chromosome
Accession NZ_CP057266
Organism Escherichia fergusonii strain RHB31-C13

Toxin (Protein)


Gene name cptA Uniprot ID F0JPE9
Locus tag HVY87_RS09285 Protein ID WP_000244774.1
Coordinates 1894148..1894555 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HVY87_RS09290 Protein ID WP_000354046.1
Coordinates 1894536..1894802 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY87_RS09265 1890105..1891838 - 1734 WP_046083279.1 single-stranded-DNA-specific exonuclease RecJ -
HVY87_RS09270 1891844..1892554 - 711 WP_000715223.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HVY87_RS09275 1892579..1893475 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HVY87_RS09280 1893587..1894108 + 522 WP_001055865.1 flavodoxin FldB -
HVY87_RS09285 1894148..1894555 - 408 WP_000244774.1 protein YgfX Toxin
HVY87_RS09290 1894536..1894802 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HVY87_RS09295 1895054..1896034 + 981 WP_000886103.1 tRNA-modifying protein YgfZ -
HVY87_RS09300 1896111..1896770 - 660 WP_000250283.1 hemolysin III family protein -
HVY87_RS09305 1896934..1897245 - 312 WP_001182952.1 N(4)-acetylcytidine aminohydrolase -
HVY87_RS09310 1897290..1898723 + 1434 WP_046083325.1 6-phospho-beta-glucosidase BglA -
HVY87_RS09315 1898771..1899664 - 894 WP_000819362.1 transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16078.05 Da        Isoelectric Point: 11.2511

>T165157 WP_000244774.1 NZ_CP057266:c1894555-1894148 [Escherichia fergusonii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T165157 NZ_CP073719:2063974-2064077 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT165157 WP_000354046.1 NZ_CP057266:c1894802-1894536 [Escherichia fergusonii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT165157 NZ_CP073719:c2064115-2063844 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L6ZX35

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References