Detailed information of TA system TA165143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3561084..3561704
• Replicon: chromosome
• Accession: NZ_CP057258
• Organism: Citrobacter freundii strain RHB31-C16

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: HVY90_RS16915
• Protein ID: WP_002892050.1
• Coordinates: 3561486..3561704 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HVY90_RS16910
• Protein ID: WP_115259201.1
• Coordinates: 3561084..3561458 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY90_RS16900	3556230..3557423	+	1194	WP_096758038.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HVY90_RS16905	3557446..3560595	+	3150	WP_096758039.1	efflux RND transporter permease AcrB	-
HVY90_RS16910	3561084..3561458	+	375	WP_115259201.1	Hha toxicity modulator TomB	Antitoxin
HVY90_RS16915	3561486..3561704	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
HVY90_RS16920	3562086..3562559	+	474	WP_096758041.1	YlaC family protein	-
HVY90_RS16925	3562633..3562773	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
HVY90_RS16930	3562775..3563035	-	261	WP_115259215.1	type B 50S ribosomal protein L31	-
HVY90_RS16935	3563220..3564773	+	1554	WP_115259216.1	EAL domain-containing protein	-
HVY90_RS16940	3564827..3565180	-	354	WP_115259217.1	DUF1428 family protein	-
HVY90_RS16945	3565263..3565874	-	612	WP_146717226.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T165143 WP_002892050.1 NZ_CP057258:3561486-3561704 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T165143 NZ_CP073718:3801454-3801795 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14454.26 Da
• Isoelectric Point: 5.5653

>AT165143 WP_115259201.1 NZ_CP057258:3561084-3561458 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSNYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT165143 NZ_CP073718:3801116-3801433 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2