Detailed information of TA system TA165134

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 836215..836876
• Replicon: chromosome
• Accession: NZ_CP057258
• Organism: Citrobacter freundii strain RHB31-C16

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: HVY90_RS04175
• Protein ID: WP_016239682.1
• Coordinates: 836553..836876 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HVY90_RS04170
• Protein ID: WP_016239683.1
• Coordinates: 836215..836532 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY90_RS04125	832141..832353	-	213	WP_001446195.1	cold shock-like protein CspF	-
HVY90_RS04130	832620..832838	+	219	WP_016239707.1	cold shock domain-containing protein	-
HVY90_RS04135	833139..833366	+	228	WP_016239708.1	hypothetical protein	-
HVY90_RS04140	833894..834298	+	405	WP_024195748.1	H-NS histone family protein	-
HVY90_RS04145	834378..834512	+	135	Protein_810	arsenical resistance protein ArsH	-
HVY90_RS04150	834810..835001	+	192	Protein_811	DUF905 family protein	-
HVY90_RS04155	835015..835476	+	462	WP_181688771.1	antirestriction protein	-
HVY90_RS04160	835485..835967	+	483	WP_181688772.1	DNA repair protein RadC	-
HVY90_RS04165	835976..836197	+	222	WP_181688773.1	DUF987 domain-containing protein	-
HVY90_RS04170	836215..836532	+	318	WP_016239683.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HVY90_RS04175	836553..836876	+	324	WP_016239682.1	TA system toxin CbtA family protein	Toxin
HVY90_RS04180	837280..837636	+	357	Protein_817	DUF4102 domain-containing protein	-
HVY90_RS04185	837675..838577	-	903	WP_181597031.1	LysR family transcriptional regulator	-
HVY90_RS04190	838707..839699	+	993	WP_181597032.1	zinc-dependent alcohol dehydrogenase family protein	-
HVY90_RS04195	839755..840747	+	993	WP_181688774.1	zinc-dependent alcohol dehydrogenase family protein	-
HVY90_RS04200	840808..840999	-	192	WP_181688775.1	gamma-glutamylcyclotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	833834..842466	8632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12359.39 Da
• Isoelectric Point: 6.4514

>T165134 WP_016239682.1 NZ_CP057258:836553-836876 [Citrobacter freundii]
MKILPATISQAAKPCLSPVTIWQMLLSRLLEQHYGLTLNDTPFCDETVIQEHIDAGITLANAINFLVEKYELVRIDRRGF
SWQEQTPYLTIIDIMRARRDLGLMNRN

Nucleotide

• Length: 324 bp

>T165134 NZ_CP073718:946133-946540 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11796.42 Da
• Isoelectric Point: 7.2625

>AT165134 WP_016239683.1 NZ_CP057258:836215-836532 [Citrobacter freundii]
MSNTIWGLQRDITPRLGARLVQEGNRLHYLADRASPTGKFSDAESLKLDVAFPHFISKMESMLTTGEMNPRHAHCVTLYY
NGFTCEADTLGCCGYVYVAVYSTQR

Nucleotide

• Length: 318 bp

>AT165134 NZ_CP073718:945886-946152 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA