Detailed information of TA system TA165108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 63614..64215
• Replicon: plasmid pRHB32-C05_4
• Accession: NZ_CP057246
• Organism: Escherichia fergusonii strain RHB32-C05

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HVY96_RS24595
• Protein ID: WP_024233187.1
• Coordinates: 63614..63994 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: HVY96_RS24600
• Protein ID: WP_001190712.1
• Coordinates: 63994..64215 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY96_RS24570	59055..60539	-	1485	WP_000124159.1	hypothetical protein	-
HVY96_RS24575	60539..61732	-	1194	WP_032167084.1	terminase	-
HVY96_RS24580	61818..62270	-	453	WP_001326849.1	late promoter-activating protein	-
HVY96_RS24585	62359..63402	-	1044	WP_024222338.1	DUF968 domain-containing protein	-
HVY96_RS24590	63430..63609	-	180	WP_000113018.1	hypothetical protein	-
HVY96_RS24595	63614..63994	-	381	WP_024233187.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HVY96_RS24600	63994..64215	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HVY96_RS24605	64288..64677	-	390	WP_181670312.1	DNA repair protein	-
HVY96_RS24610	64942..65466	-	525	WP_000159955.1	toprim domain-containing protein	-
HVY96_RS24615	65515..65715	-	201	WP_032219102.1	hypothetical protein	-
HVY96_RS24620	65827..66087	-	261	WP_181670313.1	hypothetical protein	-
HVY96_RS24625	66092..66376	-	285	WP_181670314.1	antitoxin PHD	-
HVY96_RS24630	66360..67598	-	1239	WP_181670315.1	hypothetical protein	-
HVY96_RS24635	67932..68110	-	179	Protein_72	hypothetical protein	-
HVY96_RS24640	68112..68861	-	750	WP_181670316.1	ead/Ea22-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91854	91854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13574.27 Da
• Isoelectric Point: 5.1408

>T165108 WP_024233187.1 NZ_CP057246:c63994-63614 [Escherichia fergusonii]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEDITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T165108 NZ_CP073707:c86422-86129 [Burkholderia cenocepacia]
ATGAGAATCGAGCAGACAGACGACTTCGCGAAATGGTTGCGCGGCCTACGCGACCACATCGCGCGCGCGCAGATCGCCAA
GCGGATTCAACGGCTCGCGCGCGGCCAGTACGGGGACGTGAAGTCGGTTGGTTCCGGCGTGAGCGAAATGCGCGTGCACG
TCGGCCCCGGCTATCGCGTCTACTTTGTCCAACGGGGCTCAACGCTTGTCGTCCTGCTCTGCGGTGGCGACAAGTCAACG
CAGCAGCGCGACATTGAGCGCGCTATCGCGCTTTCGACGCAATTGGGAGATTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT165108 WP_001190712.1 NZ_CP057246:c64215-63994 [Escherichia fergusonii]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT165108 NZ_CP073707:c86127-85828 [Burkholderia cenocepacia]
ATGGGTATCAAGACAACGCCGTTTGATGCGGCCGAATACCTCGACACGCCCGAGGCGCAGGCCGAATATCTGCGGATTTC
GTTTGAGACAGGCGATTCCGCCGACATTCAGGAAGCCATCGGCACCGTGGCGCGGGCGCGTGGCATGACGCAGATTGCGC
GCGACGCGGGCGTCGGCCGGGAAAGCCTCTACAAAGCGCTGGACGAGCACGGCAACCCGGAGTTCGCGACGATCCTGCGT
GTTGTGCGCGCGCTCGGCCTCACTTTGACCGTGACGACGCCCGTACACGAGCACGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6