Detailed information of TA system TA165095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 80511..81136
• Replicon: plasmid pRHB32-C05_2
• Accession: NZ_CP057244
• Organism: Escherichia fergusonii strain RHB32-C05

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HVY96_RS23235
• Protein ID: WP_000911313.1
• Coordinates: 80738..81136 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: HVY96_RS23230
• Protein ID: WP_000450520.1
• Coordinates: 80511..80738 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY96_RS23230	80511..80738	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HVY96_RS23235	80738..81136	+	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HVY96_RS23240	81145..83388	-	2244	WP_181670297.1	type IV conjugative transfer system coupling protein TraD	-
HVY96_RS23245	83439..84179	-	741	WP_151674032.1	hypothetical protein	-
HVY96_RS23250	84382..85113	-	732	WP_000850424.1	complement resistance protein TraT	-
HVY96_RS23255	85145..85642	-	498	WP_137546094.1	surface exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pefD / pefC / pefA	1..147532	147532

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T165095 WP_000911313.1 NZ_CP057244:80738-81136 [Escherichia fergusonii]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T165095 NZ_CP073702:3001121-3001444 [Burkholderia cenocepacia]
ATGAATCGAGGGAGCATTGTCACAGTCGCGCTCCAAGGTGATTTCGGCAAGGCTCGGCCGGCGCTCGTGGTGCAGTCGGA
CCTGTTTGCCGAACATCCCAGTGTAACGTTGCTGCTAATGTCGAGCGCAATGGTCGATGCGCCCTTGATACGCATTACTG
TTGTCCCATCCGAGCAGAACGGCTTGCGCATGCCGTCCCAGATTGCCGTAGACAAAATGTTCACCGTGCGCCGCGAGAAA
ATCGGTCGCCCCATCGGCCACCTCGAAGATGAACTGATGGTTGCCATCAATCGTTCGCTACTCGTTTTTCTTGGGCTAGC
GTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT165095 WP_000450520.1 NZ_CP057244:80511-80738 [Escherichia fergusonii]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT165095 NZ_CP073702:3000912-3001124 [Burkholderia cenocepacia]
ATGGCAGCTACCACTTCGGAGCGTGTTCGAATTCACCGCGAGAATCTTCGTGCGGCCGGGCTACGCCCAATTCAGATATG
GGTGCCCGACGTCCGCCAGCCGGGTTTCGCCGATGAATGCGCTCGGCAATCGCGCATGGTCCACCAGAGCATCGACGAGA
ACGATCTGCTCGATTTCATTGAACAGGCTACGGACCTCGGACACTCACAATGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2