Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 67180..67727 Replicon plasmid pRHB32-C05_2
Accession NZ_CP057244
Organism Escherichia fergusonii strain RHB32-C05

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag HVY96_RS23170 Protein ID WP_000969996.1
Coordinates 67180..67461 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag HVY96_RS23175 Protein ID WP_000079941.1
Coordinates 67458..67727 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY96_RS23155 63641..64006 + 366 WP_000124135.1 hypothetical protein -
HVY96_RS23160 64006..65021 + 1016 Protein_55 transposase -
HVY96_RS23165 65204..66700 - 1497 Protein_56 hypothetical protein -
HVY96_RS23170 67180..67461 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HVY96_RS23175 67458..67727 - 270 WP_000079941.1 hypothetical protein Antitoxin
HVY96_RS23180 68641..69498 - 858 WP_000131011.1 incFII family plasmid replication initiator RepA -
HVY96_RS23185 69491..69565 - 75 WP_001365705.1 RepA leader peptide Tap -
HVY96_RS23190 69812..70060 - 249 WP_000083838.1 replication regulatory protein RepA -
HVY96_RS23195 70344..70550 - 207 WP_001553767.1 type I toxin-antitoxin system Hok family toxin -
- 70537..70598 + 62 NuclAT_1 - -
- 70537..70598 + 62 NuclAT_1 - -
- 70537..70598 + 62 NuclAT_1 - -
- 70537..70598 + 62 NuclAT_1 - -
HVY96_RS23200 71258..71719 - 462 WP_047649930.1 thermonuclease family protein -
HVY96_RS23205 72462..72665 - 204 WP_040073114.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pefD / pefC / pefA 1..147532 147532


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T165092 WP_000969996.1 NZ_CP057244:c67461-67180 [Escherichia fergusonii]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T165092 NZ_CP073697:c862226-861900 [Edaphobacter flagellatus]
ATGGCGAGCTGGTTGGTCGAGTTCCTTGATGAAGGGGTGAAGGCATCTCTCGATGCGCTTCCCTTGGACATCCGGGCAAG
TTTTCAGCGGATCGTGGAGTTGATCCAGGCACACGGGCTGGAGCGGGTTCGGGAACCGTATGTGAAGCATCTCGAAGGCC
CGTTGTGGGAGATGCGGATGAAGGGCAAGAGTGGCATCGCACGGGCCTGTTATGTCACTGCAGTTGGGAAACGAATCGTG
GTGGTGCATGTCTTCGTAAAGAAGACACAGAAGACGCCTCGACGAGAATTGGAACTAGCCCTCAGGCGGGCGAAGGAGGT
CGAATGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT165092 WP_000079941.1 NZ_CP057244:c67727-67458 [Escherichia fergusonii]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT165092 NZ_CP073697:c861903-861589 [Edaphobacter flagellatus]
ATGAAGCGCAAGTTTATTCCGGCGGAAGAATCGTTCCGGGAGTGGAAGAAGGACCCATCGTTTGTTGCTGCATACGATGC
ACTGGAGAATGAGTTCGCTCTTGCCTCCGCGTTGATCGAGGCACGGAGCAAGGCTGATATGACTCAAGAGGAAGTTGCGG
CAGCGATGGGTACGACGCAAGCTGTTGTTGCGCGGCTTGAGAGCGGCAAGGTGTTGCCGTCTACGCGCACGCTGGAGCGG
TTCGCTCGTGCGACCCGCTCGCGGCTGCGCATCAGCTTCGAGTCCAATCAACCGGCGTTGGCTGCAAAGCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References