Detailed information of TA system TA165087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3981215..3981791
• Replicon: chromosome
• Accession: NZ_CP057243
• Organism: Escherichia fergusonii strain RHB32-C05

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HVY96_RS19415
• Protein ID: WP_181666838.1
• Coordinates: 3981504..3981791 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A7U9DAW4
• Locus tag: HVY96_RS19410
• Protein ID: WP_000063150.1
• Coordinates: 3981215..3981517 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY96_RS19390	3977029..3977541	+	513	WP_001175455.1	4-hydroxyphenylacetate 3-monooxygenase reductase subunit	-
HVY96_RS19395	3977811..3979961	+	2151	WP_001314407.1	pyruvate/proton symporter BtsT	-
HVY96_RS19400	3980011..3980214	+	204	WP_000467859.1	YbdD/YjiX family protein	-
HVY96_RS19405	3980225..3981181	+	957	WP_001295525.1	GTPase	-
HVY96_RS19410	3981215..3981517	-	303	WP_000063150.1	BrnA antitoxin family protein	Antitoxin
HVY96_RS19415	3981504..3981791	-	288	WP_181666838.1	BrnT family toxin	Toxin
HVY96_RS19420	3981988..3983154	+	1167	WP_000800829.1	restriction endonuclease	-
HVY96_RS19425	3983218..3984837	+	1620	WP_181666839.1	type I restriction-modification system subunit M	-
HVY96_RS19430	3984827..3985867	+	1041	WP_181670248.1	restriction endonuclease subunit S	-
HVY96_RS19435	3985861..3986558	-	698	Protein_3789	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3986283..3986558	275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11188.68 Da
• Isoelectric Point: 7.4697

>T165087 WP_181666838.1 NZ_CP057243:c3981791-3981504 [Escherichia fergusonii]
MPMEFEWDANKAKSNRMKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T165087 NZ_CP073677:4513807-4514235 [Cupriavidus sp. KK10]
ATGATCGTCCTGGATACGAATGTCATTTCCGAGGCCATGAAGCCGGAACCGGCTGACTCCGTGCGCTGCTGGCTCAACGA
CCAGGCCGCCGAGACCTTGTACCTGTCCAGTGTGACGCTGGCCGAACTGCTGTTCGGCATCGGCGCGCTGCCCGCGGGCA
GGCGCCGCGCCATGCTGGCCGCTGCGCTGGACGGCCTGGTGACGCTGTTCGAAGGGCGCATCTTGCCGTTCGACACGAAT
GCGGCCCGGCACTATGCGGAACTCGCCACTGCCGCCAGAAGCCGTGGCCGCGGCCTTCCCACGCCGGATGGCCACATCGC
CGCGATTGCCGCGGCCAACGGCATGATAGTCGCGACGCGGGATGTGTCGCCCTTTGAAGCGGCCGGGCTGCAGGTGCTCA
ACCCGTGGCTGGCGCCGCCTCCGTCGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11532.03 Da
• Isoelectric Point: 9.4646

>AT165087 WP_000063150.1 NZ_CP057243:c3981517-3981215 [Escherichia fergusonii]
MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT165087 NZ_CP073677:4513556-4513810 [Cupriavidus sp. KK10]
ATGCCGATCCTGACCGTTAGAAATGTGCCCGACGACGTGCACCGTGCGCTGCGGGTCCGTGCCGCCCAGAACGGACGCAG
CACTGAAGCTGAGGTGCGTGAGATCCTGGCCAGCGCGGTGATGCCGCAGAAGCGCGTGCGCCTGGGCGATGCGCTGGCGA
TGCTTGGCCGCGAGATCGGCTTGTCGAATGCGGACGTTGAAGCGATCGAGCAGGGCAAGGACAAGGCAGCGGCCGAGCCG
GTGAGGTTTGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9DAW4