Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3701984..3702638 Replicon chromosome
Accession NZ_CP057243
Organism Escherichia fergusonii strain RHB32-C05

Toxin (Protein)


Gene name cptA Uniprot ID F0JPE9
Locus tag HVY96_RS18030 Protein ID WP_000244774.1
Coordinates 3701984..3702391 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HVY96_RS18035 Protein ID WP_000354046.1
Coordinates 3702372..3702638 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY96_RS18010 3697941..3699674 - 1734 WP_000813234.1 single-stranded-DNA-specific exonuclease RecJ -
HVY96_RS18015 3699680..3700390 - 711 WP_104917537.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HVY96_RS18020 3700415..3701311 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HVY96_RS18025 3701423..3701944 + 522 WP_001055874.1 flavodoxin FldB -
HVY96_RS18030 3701984..3702391 - 408 WP_000244774.1 protein YgfX Toxin
HVY96_RS18035 3702372..3702638 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HVY96_RS18040 3702890..3703870 + 981 WP_181666750.1 tRNA-modifying protein YgfZ -
HVY96_RS18045 3703947..3704606 - 660 WP_000250283.1 hemolysin III family protein -
HVY96_RS18050 3704770..3705081 - 312 WP_001182952.1 N(4)-acetylcytidine aminohydrolase -
HVY96_RS18055 3705126..3706559 + 1434 WP_148047390.1 6-phospho-beta-glucosidase BglA -
HVY96_RS18060 3706607..3707500 - 894 WP_000819362.1 transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16078.05 Da        Isoelectric Point: 11.2511

>T165086 WP_000244774.1 NZ_CP057243:c3702391-3701984 [Escherichia fergusonii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T165086 NZ_CP073677:c3397447-3397046 [Cupriavidus sp. KK10]
ATGTCCTTGTTCCTGCTCGATACGAACATCATTTCCGACGCGATTCGCAATACCCGCGGACCCTGTGCCGCTCGTATTGC
CGCGCTCGAGCCTGACAGTCTGTGCACTTCCATCATTGTGGCGGCCGAACTCCGATTCGGAGCTGCCAAGAAAGGCTCTC
CGGCGCTGAGCCAACGTGTCGGCCAGGCTTTGGCTGGCTTGCGTGTCTTGCCGCTGGGCGGCGATGCTGACGCTTGCTAC
GGCGAGTTGCGCGCAGGACTGGAACGCAGCGGACGGGTGATTGGCGCCAATGACATGCTGATCGCGGCGCACGCTCTATC
GCTGGGTGCCGTGCTAGTGACCGACAATATCGGGGAGTTTTCTCACGTGGACGGATTGCGCTGCGAAAACTGGCTGAAGT
AG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT165086 WP_000354046.1 NZ_CP057243:c3702638-3702372 [Escherichia fergusonii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT165086 NZ_CP073677:c3397689-3397447 [Cupriavidus sp. KK10]
ATGACCACGACAGATGAAATCCGCCATGTCAGGCTGTTTCGCAATGGCCGCAATCAGGCGATCCGGATTCCGCGCGAGTT
CGAACTGCCGGGGGAGGAAGCCATTCTGCGCCGCGAAGGGAATCACCTCATCATCGAGCCGGTTGCTGCAATGCCATTGC
GTTCCTTGCTGGCGCAATGGGAGCCTATCGACGTCGATTGGCCGACGATCGATAACCTGCCTGCCGAGGATGTCGACCTC
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L6ZX35

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References