Detailed information of TA system TA165062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 136582..137225
• Replicon: plasmid pRHB32-C09_2
• Accession: NZ_CP057239
• Organism: Escherichia fergusonii strain RHB32-C09

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: C7S9Y5
• Locus tag: HVZ00_RS22960
• Protein ID: WP_001034046.1
• Coordinates: 136809..137225 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HVZ00_RS22955
• Protein ID: WP_097309516.1
• Coordinates: 136582..136812 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ00_RS22925	131818..132210	-	393	WP_112042728.1	tyrosine-type recombinase/integrase	-
HVZ00_RS22930	132211..132516	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	-
HVZ00_RS22935	132518..132736	-	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	-
HVZ00_RS22940	133323..133553	+	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HVZ00_RS22945	133550..133966	+	417	WP_097309518.1	type II toxin-antitoxin system VapC family toxin	-
HVZ00_RS22950	134128..136266	-	2139	WP_000350635.1	AAA family ATPase	-
HVZ00_RS22955	136582..136812	+	231	WP_097309516.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HVZ00_RS22960	136809..137225	+	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	Toxin
HVZ00_RS22965	137270..141063	-	3794	Protein_147	pcar	-
HVZ00_RS22970	141412..141669	+	258	WP_000343099.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyC	1..145940	145940

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14978.31 Da
• Isoelectric Point: 6.7113

>T165062 WP_001034046.1 NZ_CP057239:136809-137225 [Escherichia fergusonii]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Nucleotide

• Length: 417 bp

>T165062 NZ_CP073670:677484-677780 [Burkholderia cenocepacia]
ATGTTCAAAGTCCTGACCACTCCCCAATTTGACAAATGGCTCGGTGGTCTTCGCGACCCGGTCGGCAGCGCGGCGATCAG
CCTACGCATCGAGCGGGCGAAGCTCGGCAACCTTGGCCAGTGGCGTGCGATCGGCGACGGCGTCAACGAAATGAAGATCG
ATGTAGGGCCGGGGTACCGAGCCTACTTCGTGCGGCGCGGAAAGATAATCGTCGTGGTGTTGTGCGGTGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGACGGCGAAGCAAATCGCCGCCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8811.02 Da
• Isoelectric Point: 4.6282

>AT165062 WP_097309516.1 NZ_CP057239:136582-136812 [Escherichia fergusonii]
MRTVSIFKNGNNRAIRLPRELDFEGVSELEIIREGNSIILRPVRPTWSSFLELEKADPGFMTEREDVVCDEGRFNL

Nucleotide

• Length: 231 bp

>AT165062 NZ_CP073670:677783-678148 [Burkholderia cenocepacia]
ATGAAAATCAGCGAACTGGCCGAATTCGATGGCTCGAAGTACCTGAAGGACGAGGAAGCGATTCGTCACTACCTGGCGCA
GGCGTTTGAAGATGGAGATCCTCGACTGATCCAGGCCGCGCTCGGCAACGTCGCGAAGGCGCGCGGCATGACGGCGCTGG
CGCGCGAGTCCGGTGTGAAGCGTGAGGCGCTCTATCGCGCGCTGTCGGAGGGTGGGAACGCGGAATTTGCAACGATCATG
AAGGTCGTCGCCGCGTTGGGTTTGCATCTGACCGTTGCGCCGACCACGCAGAACGCCGAAGCGGGCCGCGGGCCTGAACC
GACCTCGCGCACGCGCTCGCACGCACGTGCGGCGGCCCACGGATGA

Structures

Toxin

• AlphaFold DB: A0A7U9NXF9