Detailed information of TA system TA165031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 133323..133966
• Replicon: plasmid pRHB32-C16_2
• Accession: NZ_CP057230
• Organism: Escherichia fergusonii strain RHB32-C16

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HVZ03_RS22945
• Protein ID: WP_097309518.1
• Coordinates: 133550..133966 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: HVZ03_RS22940
• Protein ID: WP_001261287.1
• Coordinates: 133323..133553 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ03_RS22910	128761..129927	-	1167	WP_000772446.1	plasmid-partitioning protein SopA	-
HVZ03_RS22915	130424..130597	-	174	Protein_137	RepB family plasmid replication initiator protein	-
HVZ03_RS22925	131818..132210	-	393	WP_112042728.1	tyrosine-type recombinase/integrase	-
HVZ03_RS22930	132211..132516	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	-
HVZ03_RS22935	132518..132736	-	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	-
HVZ03_RS22940	133323..133553	+	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HVZ03_RS22945	133550..133966	+	417	WP_097309518.1	type II toxin-antitoxin system VapC family toxin	Toxin
HVZ03_RS22950	134128..136266	-	2139	WP_000350635.1	AAA family ATPase	-
HVZ03_RS22955	136582..136812	+	231	WP_097309516.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HVZ03_RS22960	136809..137225	+	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyC	1..145940	145940

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15167.63 Da
• Isoelectric Point: 7.7805

>T165031 WP_097309518.1 NZ_CP057230:133550-133966 [Escherichia fergusonii]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGAPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T165031 NZ_CP073638:c2687204-2686833 [Burkholderia cepacia]
ATGATTCTTGTCGACACTTCGGTCTGGATCGATCACCTGAGAGCTGGCGATGCAACGCTCACCACGCTGCTGGAAGCCGA
ACGGGTATTGATCCATCCGTTCGTCGTCGGCGAACTTGCGCTCGGCAGCTTGCGTGATCGCGAAACCGTGCTGGATGCGC
TACGCGACCTCCCCGCCGCCGCGGCAGCAACCGATGACGAAGTTCAACGCATGATCGACGTCGCACCGCTTTACGGGCTC
GGCATCGGTTACGTCGATGCCCACCTGCTCGCGTCGGTTCGACTGACCAGCGGCAGCAAGCTGTGGACACGCGACCGCCG
TCTGCTGGCTGCGGCCGAACGCCTGCAACTCGCCGCCCACCCCGCTCACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT165031 WP_001261287.1 NZ_CP057230:133323-133553 [Escherichia fergusonii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT165031 NZ_CP073638:c2687398-2687201 [Burkholderia cepacia]
ATGCGCACCACCTTATCGCTGGACGACGCCCTGCTTGCGAAAGCGCAGCAATTGACCGGCGTCACCGAAAAATCGGCGCT
GGTCAGAGAAGCGCTGCGCGCGCTGATTGCCCGTGAAAGCGCACGACGGCTGGCTCGCCTCGGCGGCACCGAACCCGATC
TCGAATCCGTTCCGCGCCGCCCATCGGAGCCAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QFC4