Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 132211..132736 Replicon plasmid pRHB32-C16_2
Accession NZ_CP057230
Organism Escherichia fergusonii strain RHB32-C16

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HVZ03_RS22930 Protein ID WP_001159871.1
Coordinates 132211..132516 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag HVZ03_RS22935 Protein ID WP_000813639.1
Coordinates 132518..132736 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ03_RS22905 127790..128761 - 972 WP_032248840.1 ParB/RepB/Spo0J family plasmid partition protein -
HVZ03_RS22910 128761..129927 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HVZ03_RS22915 130424..130597 - 174 Protein_137 RepB family plasmid replication initiator protein -
HVZ03_RS22925 131818..132210 - 393 WP_112042728.1 tyrosine-type recombinase/integrase -
HVZ03_RS22930 132211..132516 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HVZ03_RS22935 132518..132736 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HVZ03_RS22940 133323..133553 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HVZ03_RS22945 133550..133966 + 417 WP_097309518.1 type II toxin-antitoxin system VapC family toxin -
HVZ03_RS22950 134128..136266 - 2139 WP_000350635.1 AAA family ATPase -
HVZ03_RS22955 136582..136812 + 231 WP_097309516.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HVZ03_RS22960 136809..137225 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyC 1..145940 145940


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T165030 WP_001159871.1 NZ_CP057230:c132516-132211 [Escherichia fergusonii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T165030 NZ_CP073638:2518798-2519079 [Burkholderia cepacia]
ATGACCTTTGAACTAGCCTTTCTCGAACCTGCATTGAGGGAGTGGAAGAAGCTCGATCGCACTGTTCGCGATCAATTCAA
GACCAAGCTCGCCGAGCGGCTCGAGAATCCTCGCATCCCGTCGGCCAAGTTGCATGGGCACCCGGATCGCTACAAGATCA
AGCTGCGCAGCGTCGGTTACCGACTTGTCTACGAAGTGCGCGATGCGGAAGTCGTCGTTCTCGTCGTGGCAGTCGGGCGG
CGTGAGCGCGATGCAGTCTATCTTGCCGCGATGAAACGTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT165030 WP_000813639.1 NZ_CP057230:c132736-132518 [Escherichia fergusonii]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT165030 NZ_CP073638:2518560-2518808 [Burkholderia cepacia]
ATGCCTCATACCATTCTGGCCAGCGTGACCGCGAGCGTTTCGGAACTGAAACGGAACCCGATGGGGACGGTCGCGGCAGG
AGAGGGCTTTCCGGTCGCGATTCTGAATCACAACGAGCCTGCGTTCTATTGCATTCCGGCGAAGACGTGGGAAGCCATGG
TCGAACGCCTGGAAGACATCGAAGACAATGCATTGGCCGATTCGCGCGCCAAGGAGAAGGTCGTCAAGGTCAAGCTGAAT
GACCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References