Detailed information of TA system TA165025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 83703..84328
• Replicon: plasmid pRHB32-C16_2
• Accession: NZ_CP057230
• Organism: Escherichia fergusonii strain RHB32-C16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HVZ03_RS22625
• Protein ID: WP_000911314.1
• Coordinates: 83930..84328 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: HVZ03_RS22620
• Protein ID: WP_000450532.1
• Coordinates: 83703..83930 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ03_RS22620	83703..83930	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HVZ03_RS22625	83930..84328	+	399	WP_000911314.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HVZ03_RS22630	84337..86526	-	2190	WP_181669071.1	type IV conjugative transfer system coupling protein TraD	-
HVZ03_RS22635	86779..87510	-	732	WP_000850423.1	complement resistance protein TraT	-
HVZ03_RS22640	87524..88033	-	510	WP_112042696.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyC	1..145940	145940

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14814.12 Da
• Isoelectric Point: 7.8604

>T165025 WP_000911314.1 NZ_CP057230:83930-84328 [Escherichia fergusonii]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T165025 NZ_CP073638:533107-533523 [Burkholderia cepacia]
GTGTATTTGATTGATACGAACGTCATCAGCGAAATCAGGAAGGGCAAGCGAACCAACCGTGGCGTGCGGGCGTTCTTCAG
GCAGGCCGCGGCCGACGCCAGCCCGCTTTACCTGTCCGTCGTGACAGTTGCTGAACTTCGCCGCGGCGTCGATCTGATCC
GTCATCGCGGCGATCACCCCCAGGCGTCGGCGCTCGAGGCATGGATGGCGACGATCCTGGCCGGCTATGCACCGAACATC
CTGCCGGTCGACATCGAGGTCAGCCAGATGTGGGGGCATTTGCGCGTGCCCGATCCGACGCACGAACTCGACAAGCTGAT
CGCGGCCACCGCGCTGATCAACGATCTCACCGTGGTCACGCGCAACGTCGCGGATTTTGCCCGCACGGGCGTCAGGTTGC
TGAACCCGTTCGACTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT165025 WP_000450532.1 NZ_CP057230:83703-83930 [Escherichia fergusonii]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT165025 NZ_CP073638:532884-533120 [Burkholderia cepacia]
ATGGCAAATCTACTGGTGCGTAACGTGGATGACAGTATCGTTCAGAGCCTGCGCGAGCAGGCTGCGGCAAATGGCAGGAG
TGCCGAGGCCGAACATCGGGCCATTCTGGCCGATGCGCTTGGCCGGCCGAAGCGAAAGACATTTGCGCAAGTGCTGATGA
GCATGCCCGAGGTTGGCGAAGACGCGGATTTCCAACGTGTTCAGGATTCCGGCGAGGCCAGGCGTGTATTTGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5