Detailed information of TA system TA165022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 71633..72180
• Replicon: plasmid pRHB32-C16_2
• Accession: NZ_CP057230
• Organism: Escherichia fergusonii strain RHB32-C16

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: HVZ03_RS22560
• Protein ID: WP_000969996.1
• Coordinates: 71633..71914 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q4FIL6
• Locus tag: HVZ03_RS22565
• Protein ID: WP_000079945.1
• Coordinates: 71911..72180 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ03_RS22545	67926..68291	+	366	WP_097418983.1	transposase	-
HVZ03_RS22550	68291..69474	+	1184	Protein_64	IS91 family transposase	-
HVZ03_RS22555	69657..71153	-	1497	Protein_65	hypothetical protein	-
HVZ03_RS22560	71633..71914	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVZ03_RS22565	71911..72180	-	270	WP_000079945.1	hypothetical protein	Antitoxin
HVZ03_RS22570	73094..73951	-	858	WP_019842754.1	incFII family plasmid replication initiator RepA	-
HVZ03_RS22575	73944..74426	-	483	WP_001443034.1	hypothetical protein	-
HVZ03_RS22580	74419..74493	-	75	WP_001442103.1	RepA leader peptide Tap	-
HVZ03_RS22585	74727..74984	-	258	WP_000083834.1	replication regulatory protein RepA	-
HVZ03_RS22590	75251..75457	-	207	WP_181654380.1	type I toxin-antitoxin system Hok family toxin	-
-	75444..75505	+	62	NuclAT_1	-	-
-	75444..75505	+	62	NuclAT_1	-	-
-	75444..75505	+	62	NuclAT_1	-	-
-	75444..75505	+	62	NuclAT_1	-	-
HVZ03_RS22595	75801..76112	+	312	WP_000802277.1	hypothetical protein	-
HVZ03_RS22600	76623..76835	-	213	WP_042047803.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyC	1..145940	145940

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T165022 WP_000969996.1 NZ_CP057230:c71914-71633 [Escherichia fergusonii]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T165022 NZ_CP073637:c1904278-1903982 [Burkholderia cepacia]
ATGTTCAAAGTCCTGACGACCCCTCAATTTGATAACTGGCTCGATGGGCTTCGCGACCCGGTTGGCAGCGCGGCAATCAG
CCTGCGCATCGAGCGGGCGAAGCTCGGCAACCTCGGCCAGTGGCGCGCAGTCGGTGACGGCGTTAACGAAATGAAGATCG
ACGTAGGGCCGGGATACCGTGCCTACTTCGTGCGGCGCGGAAAAATCATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGGCGGCGAAGCAAATCGCCGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10180.52 Da
• Isoelectric Point: 8.8735

>AT165022 WP_000079945.1 NZ_CP057230:c72180-71911 [Escherichia fergusonii]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT165022 NZ_CP073637:c1903979-1903617 [Burkholderia cepacia]
ATGAAAATCAGCGAACTGGCGGAATTCGACGGCTCGAAGTACCTGAAGGACGAAGAGGCGATTCGTCACTACCTGGCGCA
AGCCTTCGAAGATGGCGATCCGCGGCTGATCCAGGCCGCGCTCGGGAATGTCGCGAAAGCGCGCGGCATGACCGCGCTGG
CGCGCGAGTCCGGCGTAAAGCGCGAAGCGCTTTACCGTGCGCTGTCGGAAGGTGGGAATGCGGAGTTTGCAACGATCATG
AAGGTCGTCGGTGCGTTGGGATTGCACCTGACCGTTGCACCGACCGTGCAGGCGGCAGAAGCGGCACCGGTGCCGGCACC
GACACGCTCGCGCCCGCGTGCCCGCGCTGCGGCGCACACGTAA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: Q4FIL6