Detailed information of TA system TA165013

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1849848..1850502
• Replicon: chromosome
• Accession: NZ_CP057229
• Organism: Escherichia fergusonii strain RHB32-C16

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: F0JPE9
• Locus tag: HVZ03_RS08905
• Protein ID: WP_000244774.1
• Coordinates: 1849848..1850255 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: HVZ03_RS08910
• Protein ID: WP_181667837.1
• Coordinates: 1850236..1850502 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ03_RS08885	1845805..1847538	-	1734	WP_000813234.1	single-stranded-DNA-specific exonuclease RecJ	-
HVZ03_RS08890	1847544..1848254	-	711	WP_046076902.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HVZ03_RS08895	1848279..1849175	-	897	WP_000806645.1	site-specific tyrosine recombinase XerD	-
HVZ03_RS08900	1849287..1849808	+	522	WP_001055874.1	flavodoxin FldB	-
HVZ03_RS08905	1849848..1850255	-	408	WP_000244774.1	protein YgfX	Toxin
HVZ03_RS08910	1850236..1850502	-	267	WP_181667837.1	FAD assembly factor SdhE	Antitoxin
HVZ03_RS08915	1850754..1851734	+	981	WP_181667838.1	tRNA-modifying protein YgfZ	-
HVZ03_RS08920	1851761..1853059	+	1299	WP_181667839.1	hypothetical protein	-
HVZ03_RS08925	1853056..1853523	+	468	WP_181667840.1	hypothetical protein	-
HVZ03_RS08930	1853554..1854213	-	660	WP_000250283.1	hemolysin III family protein	-
HVZ03_RS08935	1854377..1854688	-	312	WP_001182952.1	N(4)-acetylcytidine aminohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16078.05 Da
• Isoelectric Point: 11.2511

>T165013 WP_000244774.1 NZ_CP057229:c1850255-1849848 [Escherichia fergusonii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T165013 NZ_CP073632:c1000905-1000648 [Streptococcus parasuis]
TTGAGTTATAAGGTTATTTTGTCTGCTAGGGTTCAAAAGCAAATTCGAAAAATGGATAAAACGGCTGCAAGTTTGATTTT
GCGGTATCTTTATAAGCATATCGACGGGTGCGACAACCCTAGACAATACGGAATAGCCTTAACTGCCAATCGTTCTGGCC
AATGGCGGTACCGTATCGGTAACTACAGAGTTATCGTCAGTATTGAAGATGACAAATTGATTATGACAAGCATAAAAATG
AGAAATGGAAGTATGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10531.16 Da
• Isoelectric Point: 5.6375

>AT165013 WP_181667837.1 NZ_CP057229:c1850502-1850236 [Escherichia fergusonii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSVDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT165013 NZ_CP073632:c1001134-1000895 [Streptococcus parasuis]
ATGGCAGCTATTACATTAAAAGTTTCTGAGCAAGACAAGCTATTTATGCAGGCTATGGCAAAGTTTGAAGGTGTCAGCTT
GTCAGAACTCATCCGGACTAAAACCCTTGCAGCTCTCGAAGATGAATACGATGCCCGCGTGGCAGATATTGCTTGGGCAG
AATACCAAGCTGACTTGGCGAATGGAATTGAGCCGATAACACTAGAACAAATGGCTGAGGAGTTGGGCATTGAGTTATAA

Structures

Toxin

• AlphaFold DB: A0A0L6ZX35