Detailed information of TA system TA165002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 144721..145315
• Replicon: plasmid pRHB32-C22_2
• Accession: NZ_CP057221
• Organism: Escherichia fergusonii strain RHB32-C22

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HVZ07_RS22815
• Protein ID: WP_001403920.1
• Coordinates: 145040..145315 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: G3CAJ1
• Locus tag: HVZ07_RS22810
• Protein ID: WP_001261274.1
• Coordinates: 144721..144951 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ07_RS22795	140224..140996	-	773	Protein_147	hypothetical protein	-
HVZ07_RS22800	141025..141510	-	486	WP_001403958.1	hypothetical protein	-
HVZ07_RS22805	141901..144522	+	2622	WP_000283688.1	histidinol-phosphatase	-
HVZ07_RS22810	144721..144951	+	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HVZ07_RS22815	145040..145315	+	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	Toxin
HVZ07_RS22820	145320..145537	-	218	Protein_152	transposase	-
HVZ07_RS22825	145538..146068	-	531	WP_053285705.1	GNAT family N-acetyltransferase	-
HVZ07_RS22830	146072..146341	-	270	WP_001275013.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyC	1..147240	147240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 9621.03 Da
• Isoelectric Point: 8.5444

>T165002 WP_001403920.1 NZ_CP057221:145040-145315 [Escherichia fergusonii]
VFSHRIVVSAITYSEMRFGASGPKASPRHVQLVDKFCPCLDAILSWDRAAVDGTTDIKVALRQAGTPIGQNDTAIAGPEA
GRLGKLTSPTL

Nucleotide

• Length: 276 bp

>T165002 NZ_CP073627:c4718451-4718167 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8856.95 Da
• Isoelectric Point: 4.6286

>AT165002 WP_001261274.1 NZ_CP057221:144721-144951 [Escherichia fergusonii]
MRTVSIFKNGNNRAIHLPRDLDFERVRELEIVREGDSIILRPVQPNWGSFAQLEKADPDFMAERENVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT165002 NZ_CP073627:c4718683-4718441 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGGTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: G3CAJ1