Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 131048..131573 Replicon plasmid pRHB32-C22_2
Accession NZ_CP057221
Organism Escherichia fergusonii strain RHB32-C22

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag HVZ07_RS22750 Protein ID WP_001159868.1
Coordinates 131048..131353 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HVZ07_RS22755 Protein ID WP_000813634.1
Coordinates 131355..131573 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ07_RS22730 126110..127081 - 972 WP_000817031.1 ParB/RepB/Spo0J family plasmid partition protein -
HVZ07_RS22735 127081..128247 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HVZ07_RS22740 128835..129590 - 756 WP_000852146.1 replication initiation protein RepE -
HVZ07_RS22745 130241..131047 - 807 WP_000016982.1 site-specific integrase -
HVZ07_RS22750 131048..131353 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
HVZ07_RS22755 131355..131573 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HVZ07_RS22760 132281..133276 + 996 WP_000246635.1 hypothetical protein -
HVZ07_RS22765 133280..134212 + 933 WP_000991830.1 hypothetical protein -
HVZ07_RS22770 134510..135598 + 1089 WP_000952231.1 hypothetical protein -
HVZ07_RS22775 135600..136469 + 870 WP_000253407.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyC 1..147240 147240


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T165001 WP_001159868.1 NZ_CP057221:c131353-131048 [Escherichia fergusonii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T165001 NZ_CP073627:c4625780-4625439 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT165001 WP_000813634.1 NZ_CP057221:c131573-131355 [Escherichia fergusonii]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT165001 NZ_CP073627:c4626142-4625801 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References